At2g34650.1

In the plant Arabidopsis thaliana, At2g34650.1 is a gene model for the locus At2g34650.^[1]

Also known as ABR, and PID, this gene model is classified by TAIR as "PID (PINOID); kinase". It is located on chr2, region 14597013-14598636 REVERSE.

Physiological function[]

At2g34650.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At2g34650.1/Expression

Subcellular localization[]

At2g34650.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At2g34650.1 has 438 amino-acid residues and a molecular weight Mw = 49.283 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 12.89, or 0.029 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At2g34650.1/Knockout

Protein interactions[]

At2g34650.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At2g34650.1 has 1 recognized domains:

Domain_0075_0394 Pkinase domain (PF00069, IPR000719) Description: Protein kinase;Molecular Function: protein kinase activity (GO:0004672), Molecular Function: ATP binding (GO:0005524), Biological Process: protein amino acid phosphorylation (GO:0006468)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At2g34650.1 MLRESDGEMS LGTTNSPISS GTESCSSFSR LSFDAPPSTI PEEESFLSLK PHRSSDFAYA EIRRRKKQGL TFRDFRLMRR IGAGDIGTVY LCRLAGDEEE SRSSYFAMKV VDKEALALKK KMHRAEMEKT ILKMLDHPFL PTLYAEFEAS HFSCIVMEYC SGGDLHSLRH RQPHRRFSLS SARFYAAEVL VALEYLHMLG IIYRDLKPEN ILVRSDGHIM LSDFDLSLCS DSIAAVESSS SSPENQQLRS PRRFTRLARL FQRVLRSKKV QTLEPTRLFV AEPVTARSGS FVGTHEYVAP EVASGGSHGN AVDWWAFGVF LYEMIYGKTP FVAPTNDVIL RNIVKRQLSF PTDSPATMFE LHARNLISGL LNKDPTKRLG SRRGAAEVKV HPFFKGLNFA LIRTLTPPEI PSSVVKKPMK SATFSGRSSN KPAAFDYF ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-