In the plant Arabidopsis thaliana, At1g01340.1 is a gene model for the locus At1g01340.[1]
At1g01340.1/Header
Physiological function[]
At1g01340.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g01340.1/Expression
Subcellular localization[]
At1g01340.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g01340.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g01340.1/Knockout
Protein interactions[]
At1g01340.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g01340.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g01340.1
MILFRFKDEG
KPLSSEYGYG
RKARPSLDRV
FKNVKWGFKK
PLSFPSHKDP
DHKETSSVTR
KNIINPQDSF
LQNWNKIFLF
ACVVALAIDP
LFFYIPIVDS
ARHCLTLDSK
LEIAASLLRT
LIDAFYIIHI
VFQFRTAYIA
PSSRVFGRGE
LVDDAKAIAL
KYLSSYFIID
LLSILPLPQI
VVLAVIPSVN
QPVSLLTKDY
LKFSIIAQYV
PRILRMYPLY
TEVTRTSGIV
TETAWAGAAW
NLSLYMLASH
VFGALWYLIS
VEREDRCWQE
ACEKTKGCNM
KFLYCENDRN
VSNNFLTTSC
PFLDPGDITN
STIFNFGIFT
DALKSGVVES
HDFWKKFFYC
FWWGLRNLSA
LGQNLQTSKF
VGEIIFAISI
CISGLVLFAL
LIGNMQKYLE
STTVREEEMR
VRKRDAEQWM
SHRMLPEDLR
KRIRRYEQYR
WQETRGVEEE
TLLRNLPKDL
RRDIKRHLCL
DLLKKVPLFE
IMDEQLLDAV
CDRLRPVLYT
ENSYVIREGD
PVGEMLFVMR
GRLVSATTNG
GRSGFFNAVN
LKASDFCGED
LLPWALDPQS
SSHFPISTRT
VQALTEVEAF
ALTAEDLKSV
ASQFRRLHSK
QLQHTFRFYS
VQWRTWSVSF
IQAAWRRYCR
RKLAKSLRDE
EDRLREALAS
QDKEHNAATV
SSSLSLGGAL
YASRFASNAL
HNLRHNISNL
PPRYTLPLLP
QKPTEPDFTA
NHTTDP
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.