In the plant Arabidopsis thaliana, At2g44590.3 is a gene model for the locus At2g44590.[1]
At2g44590.3/Header
Physiological function[]
At2g44590.3/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At2g44590.3/Expression
Subcellular localization[]
At2g44590.3/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At2g44590.3/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At2g44590.3/Knockout
Protein interactions[]
At2g44590.3/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At2g44590.3/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At2g44590.3
MESLIVLINT
IQRACTVVGD
HGGDSNALSS
LWEALPSVAV
VGGQSSGKSS
VLESIVGRDF
LPRGSGIVTR
RPLVLQLHKT
ENGTEDNAEF
LHLTNKKFTN
FSLVRKEIED
ETDRITGKNK
QISSIPIHLS
IFSPNVVNLT
LIDLPGLTKV
AVEGQPETIV
EDIESMVRSY
VEKPNCLILA
ISPANQDIAT
SDAMKLAKEV
DPIGDRTFGV
LTKLDLMDKG
TNALDVINGR
SYKLKYPWVG
IVNRSQADIN
KNVDMMVARR
KEREYFETSP
DYGHLATRMG
SEYLAKLLSK
LLESVIRSRI
PSILSLINNN
IEELERELDQ
LGRPIAIDAG
AQLYTILGMC
RAFEKIFKEH
LDGGRPGGAR
IYGIFDYNLP
TAIKKLPFDR
HLSLQSVKRI
VSESDGYQPH
LIAPELGYRR
LIEGSLNHFR
GPAEASVNAI
HLILKELVRK
AIAETEELKR
FPSLQIELVA
AANSSLDKFR
EESMKSVLRL
VDMESSYLTV
DFFRKLHVES
QNMSLSSPTS
AIDQYGDGHF
RKIASNVAAY
IKMVAETLVN
TIPKAVVHCQ
VRQAKLSLLN
YFYAQISQSQ
GKRLGQLLDE
NPALMERRMQ
CAKRLELYKK
ARDEIDAAVW
VR
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.