At1g10290.1

In the plant Arabidopsis thaliana, At1g10290.1 is a gene model for the locus At1g10290.^[1]

At1g10290.1/Header

Physiological function[]

At1g10290.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g10290.1/Expression

Subcellular localization[]

At1g10290.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

At1g10290.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g10290.1/Knockout

Protein interactions[]

At1g10290.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

At1g10290.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g10290.1 MEAIDELSQL SDSMKQAASL LADEDPDETS SSKRPATFLN VVALGNVGAG KSAVLNSLIG HPVLPTGENG ATRAPIIIEL SRESSLSSKA IILQIDNKSQ QVSASALRHS LQDRLSKGAS GKNRDEINLK LRTSTAPPLK LVDLPGLDQR IVDESMIAEY AQHNDAILLV IVPASQASEI SSSRALKIAK EYDPESTRTI GIIGKIDQAA ENSKALAAVQ ALLSNQGPPK TTDIPWVAVI GQSVSIASAQ SGSGENSLET AWRAESESLK SILTGAPQSK LGRIALVDTL ASQIRSRMKL RLPSVLSGLQ GKSQIVQDEL ARLGEQLVNS AEGTRAIALE LCREFEDKFL LHLAGGEGSG WKVVASFEGN FPNRIKQLPL DRHFDLNNVK RVVLEADGYQ PYLISPEKGL RSLIKIVLEL AKDPARLCVD EVHRVLVDIV SASANATPGL GRYPPFKREV VAIASAALDG FKNEAKKMVV ALVDMERAFV PPQHFIRLVQ RRMERQRREE ELKGRSSKKG QDAEQSLLSR ATSPQPDGPT AGGSLKSMKD KPSPQDKETP EVSGLKTAGP EGEITAGYLM KKSAKTNGWS RRWFVLNEKT GKLGYTKKQE ERNFRGTITL EECTIEEIPE DEVEKSKSSK DKKANGPDSK GPGLVFKITC KVPYKTVLKA HNALVLKAES VVDKNEWINK LQKVIQARGG QVGSVSMRQS LSEGSLDKMV RKPIDPEEEL RWMSQEVRGY VEAVLNSLAA NVPKAVVLCQ VEKAKEDMLN QLYSSISAIG NERIESLIQE DQNVKRRRER YQKQSSLLSK LTRQLSIHDN RAAAASSYSD NSGTESSPRA SGGSSGDDWM NAFNSAANGP SDSLSKYGSG GHSRRYSDPA QNGDAASPGS GSNRRTTPNR LPPAPPPTGS AYRY ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-