In the plant Arabidopsis thaliana, At2g24520.1 is a gene model for the locus At2g24520.[1]
At2g24520.1/Header
Physiological function[]
At2g24520.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At2g24520.1/Expression
Subcellular localization[]
At2g24520.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At2g24520.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At2g24520.1/Knockout
Protein interactions[]
At2g24520.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At2g24520.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At2g24520.1
MEEVFEELKC
TKQGLTANEA
SHRLDVFGPN
KLEEKKESKL
LKFLGFMWNP
LSWVMEVAAL
MAIALANGGG
RPPDWQDFVG
IVCLLLINST
ISFIEENNAG
NAAAALMAGL
APKTKVLRDN
QWSEQEASIL
VPGDVISIKL
GDIIPADARL
LDGDPLKIDQ
SSLTGESIPV
TKNPSDEVFS
GSICKQGEIE
AIVIATGVHT
FFGKAAHLVD
NTNQIGHFQK
VLTSIGNFCI
CSIALGIIVE
LLVMYPIQRR
RYRDGIDNLL
VLLIGGIPIA
MPSVLSVTMA
TGSHRLFQQG
AITKRMTAIE
EMAGMDVLCC
DKTGTLTLNK
LTVDKNLVEV
FAKGVGKEHV
FLLAARASRI
ENQDAIDAAI
VGMLADPKEA
RAGVREVHFF
PFNPVDKRTA
LTYVDSDGNW
HRASKGAPEQ
ILNLCNCKED
VRRKVHGVID
KFAERGLRSL
AVARQEVLEK
KKDAPGGPWQ
LVGLLPLFDP
PRHDSAETIR
RALNLGVNVK
MITGDQLAIG
KETGRRLGMG
TNMYPSSALL
GQVKDSSLGA
LPVDELIEKA
DGFAGVFPEH
KYEIVHRLQQ
RNHICGMTGD
GVNDAPALKK
ADIGIAVVDA
TDAARGASDI
VLTEPGLSVI
ISAVLTSRAI
FQRMKNYTIY
AVSITIRIVF
GFMFIALIWQ
FDFSPFMVLI
IAILNDGTIM
TISKDRMKPS
PQPDSWKLRD
IFSTGVVLGG
YQALMTVVFF
WVMKDSDFFS
NYFGVRPLSQ
RPEQMMAALY
LQVSIISQAL
IFVTRSRSWS
YAECPGLLLL
GAFVIAQLVA
TFIAVYANWS
FARIEGAGWG
WAGVIWLYSF
LTYIPLDLLK
FGIRYVLSGK
AWLNLLENKT
AFTTKKDYGK
EEREAQWAAA
QRTLHGLQPA
EKNNIFNEKN
SYSELSQIAE
QAKRRAEVVR
LREINTLKGH
VESVVKLKGL
DIDTIQQHYT
V
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.