At1g01080.2

In the plant Arabidopsis thaliana, At1g01080.2 is a gene model for the locus At1g01080.^[1]

This gene model is classified by TAIR as "RNA binding". It is located on chr1, region 45503-46789 REVERSE.

Physiological function[]

At1g01080.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g01080.2/Expression

Subcellular localization[]

At1g01080.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g01080.2 has 294 amino-acid residues and a molecular weight Mw = 32.680 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 5.33, or 0.018 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g01080.2/Knockout

Protein interactions[]

At1g01080.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g01080.2 has 2 recognized domains:

Domain_0110_0182 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)

Domain_0215_0285 RRM_1 domain (PF00076, IPR000504) Description: RNA-binding region RNP-1 (RNA recognition motif);Molecular Function: nucleic acid binding (GO:0003676)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g01080.2 MAASCFAIPL SSSSRSSHNA IPKYKTLISS SSYSYLESLK LQFSSSNSFH HSSLSRPFVA QPLQIKVSSS ELSVLDEEKE EEVVKGEAEP NKDSVVSKAE PVKKPRPCEL YVCNIPRSYD IAQLLDMFQP FGTVISVEVV SRNPQTGESR GSGYVTMGSI NSAKIAIASL DGTEVGGREM RVRYSVDMNP GTRRNPEVLN STPKKILMYE SQHKVYVGNL PWFTQPDGLR NHFSKFGTIV STRVLHDRKT GRNRVFAFLS FTSGEERDAA LSFNGTQYEG RRIIVREGIE KSES ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-