At1g01220.1

In the plant Arabidopsis thaliana, At1g01220.1 is a gene model for the locus At1g01220.

At1g01220.1/Header

Physiological function

At1g01220.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At1g01220.1/Expression

Subcellular localization

At1g01220.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

At1g01220.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At1g01220.1/Knockout

Protein interactions

At1g01220.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

At1g01220.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At1g01220.1
MSKQRKKADL ATVLRKSWYH LRLSVRHPTR VPTWDAIVLT AASPEQAELY DWQLRRAKRM GRIASSTVTL AVPDPDGKRI GSGAATLNAI YALARHYEKL GFDLGPEMEV ANGACKWVRF ISAKHVLMLH AGGDSKRVPW ANPMGKVFLP LPYLAADDPD GPVPLLFDHI LAIASCARQA FQDQGGLFIM TGDVLPCFDA FKMTLPEDAA SIVTVPITLD IASNHGVIVT SKSESLAESY TVSLVNDLLQ KPTVEDLVKK DAILHDGRTL LDTGIISARG RAWSDLVALG CSCQPMILEL IGSKKEMSLY EDLVAAWVPS RHDWLRTRPL GELLVNSLGR QKMYSYCTYD LQFLHFGTSS EVLDHLSGDA SGIVGRRHLC SIPATTVSDI AASSVILSSE IAPGVSIGED SLIYDSTVSG AVQIGSQSIV VGIHIPSEDL GTPESFRFML PDRHCLWEVP LVGHKGRVIV YCGLHDNPKN SIHKDGTFCG KPLEKVLFDL GIEESDLWSS YVAQDRCLWN AKLFPILTYS EMLKLASWLM GLDDSRNKEK IKLWRSSQRV SLEELHGSIN FPEMCNGSSN HQADLAGGIA KACMNYGMLG RNLSQLCHEI LQKESLGLEI CKNFLDQCPK FQEQNSKILP KSRAYQVEVD LLRACGDEAK AIELEHKVWG AVAEETASAV RYGFREHLLE SSGKSHSENH ISHPDRVFQP RRTKVELPVR VDFVGGWSDT PPWSLERAGY VLNMAITLEG SLPIGTIIET TNQMGISIQD DAGNELHIED PISIKTPFEV NDPFRLVKSA LLVTGIVQEN FVDSTGLAIK TWANVPRGSG LGTSSILAAA VVKGLLQISN GDESNENIAR LVLVLEQLMG TGGGWQDQIG GLYPGIKFTS SFPGIPMRLQ VVPLLASPQL ISELEQRLLV VFTGQVRLAH QVLHKVVTRY LQRDNLLISS IKRLTELAKS GREALMNCEV DEVGDIMSEA WRLHQELDPY CSNEFVDKLF EFSQPYSSGF KLVGAGGGGF SLILAKDAEK AKELRQRLEE HAEFDVKVYN WSICI ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At1g01220

• PubMed Central references for At1g01220

• Google Scholar references for At1g01220

General database links

• TAIR Locus At1g01220

• TIGR page for At1g01220

• KEGG page for At1g01220

• SWISS-PROT entry for At1g01220

• Structural model of At1g01220

• NCBI structural data for At1g01220

• NCBI Entrez for At1g01220

• Gramene page for At1g01220

• MIPS MAtDB gene page for At1g01220

• MIPS SESAM alignment for At1g01220

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At1g01220

• NCBI protein data for At1g01220

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At1g01220