In the plant Arabidopsis thaliana, At1g01830.1 is a gene model for the locus At1g01830.[1]
At1g01830.1/Header
Physiological function[]
At1g01830.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g01830.1/Expression
Subcellular localization[]
At1g01830.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
At1g01830.1/Biophysics_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g01830.1/Knockout
Protein interactions[]
At1g01830.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
At1g01830.1/Domains_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g01830.1
MGTMAELGTM
GKFGAMAEEQ
QVNQMIMDKQ
SVEEWLSRVN
SLIPSVLSKA
KTVKKFTGRW
KTIISKIEQI
PACLSDLSSH
PCFSKNKLCN
EQLQSVAKTL
SEVIELAEQC
STDKYEGKLR
MQSDLDSLSG
KLDLNLRDCG
VLIKTGVLGE
ATLPLYISSS
SETPKISSLK
ELLARLQIGH
LESKHNALES
LLGAMQEDEK
MVLMPLIGRA
NVAALVQLLT
ATSTRIREKA
VNLISVLAES
GHCDEWLISE
GVLPPLVRLI
ESGSLETKEK
AAIAIQRLSM
TEENAREIAG
HGGITPLIDL
CKTGDSVSQA
ASAAALKNMS
AVSELRQLLA
EEGIIRVSID
LLNHGILLGS
REHMAECLQN
LTAASDALRE
AIVSEGGVPS
LLAYLDGPLP
QQPAVTALRN
LIPSVNPEIW
VALNLLPRLR
HVLKSGSLGA
QQAAASAICR
FACSPETKRL
VGESGCIPEI
VKLLESKSNG
CREAAAQAIA
GLVAEGRIRR
ELKKDGKSVL
TNLVMLLDSN
PGNTAKKYAV
AGLLGMSGSE
KSKKMMVSYG
AIGYLKKLSE
MEVMGADKLL
EKLERGKLRS
FFHR
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.