In the plant Arabidopsis thaliana, At1g01950.1 is a gene model for the locus At1g01950.[1]
This gene model is classified by TAIR as "armadillo/beta-catenin repeat family protein / kinesin motor family protein". It is located on chr1, region 325473-330403 FORWARD.
Physiological function[]
At1g01950.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g01950.1/Expression
Subcellular localization[]
At1g01950.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g01950.1 has 894 amino-acid residues and a molecular weight Mw = 98.417 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -3.07, or -0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g01950.1/Knockout
Protein interactions[]
At1g01950.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g01950.1 has 4 recognized domains:
- Domain_0066_0403 Kinesin domain (PF00225, IPR001752) Description: Kinesin, motor region;Molecular Function: microtubule motor activity (GO:0003777), Molecular Function: ATP binding (GO:0005524), Cellular Component: microtubule associated complex (GO:0005875), Biological Process: microtubule-based movement (GO:0007018)
- Domain_0750_0790 Arm domain (PF00514, IPR000225) Description: Armadillo_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g01950.1
MAMASSRNGA
VRGSMRPVSG
ANSSNLRSSS
FKSRIPSSAP
APRRSSSASI
GAADNGVPGR
VRVAVRLRPR
NADESVADAD
FADCVELQPE
LKRLKLRKNN
WDTETYEFDE
VLTEAASQKR
VYEVVAKPVV
ESVLEGYNGT
VMAYGQTGTG
KTFTLGRLGD
EDTAARGIMV
RSMEDIIGGT
SLDTDSISVS
YLQLYMETIQ
DLLDPTNDNI
AIVEDPRTGD
VSLPGATHVE
IRNQQNFLEL
LQLGETHRVA
ANTKLNTESS
RSHAILMVHV
KRSVVENEFP
VSNEMESSSH
FVRPSKPLVR
RSKLVLVDLA
GSERVHKSGS
EGHMLEEAKS
INLSLSALGK
CINAIAENSP
HVPLRDSKLT
RLLRDSFGGT
ARTSLIVTIG
PSPRHRGETT
STILFGQRAM
KVENMLKIKE
EFDYKSLSKK
LEVQLDKVIA
ENERQLKAFD
DDVERINRQA
QNRISEVEKN
FAEALEKEKL
KCQMEYMESV
KKLEEKLISN
QRNHENGKRN
GEVNGVVTAS
EFTRLKESLE
NEMKLRKSAE
EEVSKVKSQS
TLKTRSGEGE
DAGITRLQKL
LEDEALQKKK
LEEEVTILRS
QLVQLTFEAD
QMRRCLDRGA
PGNSYSGTDS
LPSRHSQARE
SVNGQKAPFA
TLCEQVGLQK
ILQLLESDDA
NIRIHAVKVV
ANLAAEEANQ
EKIVEAGGLT
SLLMLLRSYE
DETVRRVAAG
AIANLAMNEV
SQQLIVDQGG
ISLLSLTAAD
AEDPQTLRMV
AGAIANLCGN
DKLQARLWSD
GGIKALLGMV
RCGHPDVLAQ
VARGIANFAK
CESRATTQGV
KSGRSLLIED
GALPWIVQHA
NDEAAPIRRH
IELALCHLAQ
HEVNAKEMIS
GGALWELVRI
SKECSREDIR
SLAHRTLSSS
PVFRSEIRRL
GIQF
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.