At1g02170.1

In the plant Arabidopsis thaliana, At1g02170.1 is a gene model for the locus At1g02170.^[1]

Also known as ATMCPB1, MCP1B, AMC1, and LOL3, this gene model is classified by TAIR as "LOL3 (LSD ONE LIKE 3); caspase/ cysteine-type endopeptidase". It is located on chr1, region 411883-413426 FORWARD.

Physiological function[]

At1g02170.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g02170.1/Expression

Subcellular localization[]

At1g02170.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g02170.1 has 367 amino-acid residues and a molecular weight Mw = 39.792 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.24, or -0.001 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g02170.1/Knockout

Protein interactions[]

At1g02170.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g02170.1 has 2 recognized domains:

Domain_0018_0042 zf-LSD1 domain (PF06943, IPR005735) Description: Zinc finger, LSD1-type

Domain_0080_0363 Peptidase_C14 domain (PF00656, IPR011600) Description: Peptidase C14, caspase catalytic;Biological Process: proteolysis (GO:0006508), Molecular Function: caspase activity (GO:0030693)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g02170.1 MYPPPPSSIY APPMLVNCSG CRTPLQLPSG ARSIRCALCQ AVTHIADPRT APPPQPSSAP SPPPQIHAPP GQLPHPHGRK RAVICGISYR FSRHELKGCI NDAKCMRHLL INKFKFSPDS ILMLTEEETD PYRIPTKQNM RMALYWLVQG CTAGDSLVFH YSGHGSRQRN YNGDEVDGYD ETLCPLDFET QGMIVDDEIN ATIVRPLPHG VKLHSIIDAC HSGTVLDLPF LCRMNRAGQY VWEDHRPRSG LWKGTAGGEA ISISGCDDDQ TSADTSALSK ITSTGAMTFC FIQAIERSAQ GTTYGSLLNS MRTTIRNTGN DGGGSGGVVT TVLSMLLTGG SAIGGLRQEP QLTACQTFDV YAKPFTL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-