At1g02680.1

In the plant Arabidopsis thaliana, At1g02680.1 is a gene model for the locus At1g02680.^[1]

This gene model is classified by TAIR as "transcription initiation factor IID (TFIID) 18 kDa subunit (TAFII-18) family protein". It is located on chr1, region 581084-581919 FORWARD.

Physiological function[]

At1g02680.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g02680.1/Expression

Subcellular localization[]

At1g02680.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g02680.1 has 126 amino-acid residues and a molecular weight Mw = 14.252 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -1.89, or -0.015 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g02680.1/Knockout

Protein interactions[]

At1g02680.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g02680.1 has 1 recognized domains:

Domain_0029_0119 TFIID-18kDa domain (PF02269, IPR003195) Description: Transcription initiation factor IID, 18 kDa subunit;Molecular Function: RNA polymerase II transcription factor activity (GO:0003702), Cellular Component: transcription factor complex (GO:0005667), Biological Process: transcription initiation (GO:0006352)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g02680.1 MSNTPAAAAS SSSKSKAAGT SQPQEKRKTL FQKELQHMMY GFGDEQNPLP ESVALVEDIV VEYVTDLTHK AQEIGSKRGR LLVDDFLYLI RKDLPKLNRC RELLAMQEEL KQARKAFDVD EKELVD ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-