At1g02730.1

In the plant Arabidopsis thaliana, At1g02730.1 is a gene model for the locus At1g02730.

Also known as CSLD5, and ATCSLD5, this gene model is classified by TAIR as "ATCSLD5 (Cellulose synthase-like D5); cellulose synthase". It is located on chr1, region 594697-598473 REVERSE.

Physiological function

At1g02730.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At1g02730.1/Expression

Subcellular localization

At1g02730.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

The gene model At1g02730.1 has 1181 amino-acid residues and a molecular weight Mw = 132.589 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.31, or 0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At1g02730.1/Knockout

Protein interactions

At1g02730.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

The gene model At1g02730.1 has 1 recognized domains:

• Domain_0302_1181 Cellulose_synt domain (PF03552, IPR005150) Description: Cellulose synthase;Cellular Component: membrane (GO:0016020), Molecular Function: cellulose synthase (UDP-forming) activity (GO:0016760), Biological Process: cellulose biosynthesis (GO:0030244)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At1g02730.1
MVKSAASQSP SPVTITVTPC KGSGDRSLGL TSPIPRASVI TNQNSPLSSR ATRRTSISSG NRRSNGDEGR YCSMSVEDLT AETTNSECVL SYTVHIPPTP DHQTVFASQE SEEDEMLKGN SNQKSFLSGT IFTGGFKSVT RGHVIDCSMD RADPEKKSGQ ICWLKGCDEK VVHGRCECGF RICRDCYFDC ITSGGGNCPG CKEPYRDIND DPETEEEDEE DEAKPLPQMG ESKLDKRLSV VKSFKAQNQA GDFDHTRWLF ETKGTYGYGN AVWPKDGYGI GSGGGGNGYE TPPEFGERSK RPLTRKVSVS AAIISPYRLL IALRLVALGL FLTWRVRHPN REAMWLWGMS TTCELWFALS WLLDQLPKLC PVNRLTDLGV LKERFESPNL RNPKGRSDLP GIDVFVSTAD PEKEPPLVTA NTILSILAVD YPVEKLACYL SDDGGALLTF EALAQTASFA STWVPFCRKH NIEPRNPEAY FGQKRNFLKN KVRLDFVRER RRVKREYDEF KVRINSLPEA IRRRSDAYNV HEELRAKKKQ MEMMMGNNPQ ETVIVPKATW MSDGSHWPGT WSSGETDNSR GDHAGIIQAM LAPPNAEPVY GAEADAENLI DTTDVDIRLP MLVYVSREKR PGYDHNKKAG AMNALVRTSA IMSNGPFILN LDCDHYIYNS MALREGMCFM LDRGGDRICY VQFPQRFEGI DPNDRYANHN TVFFDVSMRA LDGLQGPMYV GTGCIFRRTA LYGFSPPRAT EHHGWLGRRK VKISLRRPKA MMKKDDEVSL PINGEYNEEE NDDGDIESLL LPKRFGNSNS FVASIPVAEY QGRLIQDLQG KGKNSRPAGS LAVPREPLDA ATVAEAISVI SCFYEDKTEW GKRVGWIYGS VTEDVVTGYR MHNRGWRSIY CVTKRDAFRG TAPINLTDRL HQVLRWATGS VEIFFSRNNA IFATRRMKFL QRVAYFNVGM YPFTSLFLIV YCILPAISLF SGQFIVQSLD ITFLIYLLSI TLTLCMLSLL EIKWSGITLH EWWRNEQFWV IGGTSAHPAA VLQGLLKVIA GVDISFTLTS KSSAPEDGDD EFADLYVVKW SFLMVPPLTI MMVNMIAIAV GLARTLYSPF PQWSKLVGGV FFSFWVLCHL YPFAKGLMGR RGRVPTIVFV WSGLLSIIVS LLWVYINPPS GKQDYMQFQF P ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At1g02730

• PubMed Central references for At1g02730

• Google Scholar references for At1g02730

General database links

• TAIR Locus At1g02730

• TIGR page for At1g02730

• KEGG page for At1g02730

• SWISS-PROT entry for At1g02730

• Structural model of At1g02730

• NCBI structural data for At1g02730

• NCBI Entrez for At1g02730

• Gramene page for At1g02730

• MIPS MAtDB gene page for At1g02730

• MIPS SESAM alignment for At1g02730

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At1g02730

• NCBI protein data for At1g02730

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At1g02730