At1g03475.1

In the plant Arabidopsis thaliana, At1g03475.1 is a gene model for the locus At1g03475.^[1]

Also known as HEMF1, ATCPO-I, and LIN2, this gene model is classified by TAIR as "LIN2 (LESION INITIATION 2); coproporphyrinogen oxidase". It is located on chr1, region 869458-871176 REVERSE.

Physiological function[]

At1g03475.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g03475.1/Expression

Subcellular localization[]

At1g03475.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g03475.1 has 362 amino-acid residues and a molecular weight Mw = 40.762 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.44, or 0.004 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g03475.1/Knockout

Protein interactions[]

At1g03475.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g03475.1 has 1 recognized domains:

Domain_0077_0360 Coprogen_oxidas domain (PF01218, IPR001260) Description: Coproporphyrinogen III oxidase;Molecular Function: coproporphyrinogen oxidase activity (GO:0004109), Biological Process: porphyrin biosynthesis (GO:0006779)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g03475.1 MASHSSTLLS SPTFAPFSSH RLHYSPNPST LRFSRPIRNK PNLALRCSVS IEKEVPETER PFTFLRDSDD VTPSSSSSSV RARFETMIRA AQDSVCDAIE AIEGGPKFKE DVWSRPGGGG GISRVLQDGN VFEKAGVNVS VVYGVMPPEA YRAAKGSASD QKPGPVPFFA AGVSSVLHPK NPFAPTLHFN YRYFETDAPK DVPGAPRQWW FGGGTDFTPA YIFEEDVKHF HSIQKQACDK FDPSFYPRFK KWCDDYFYIK HRDERRGLGG IFFDDLNDYD QEMLLSFATE CANSVVPAYI PIVEKRKDME FTEQHKAWQQ LRRGRYVEFN LVYDRGTTFG LKTGGRIESI LVSLPAFSKM GI ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-