In the plant Arabidopsis thaliana, At1g03510.1 is a gene model for the locus At1g03510.[1]
This gene model is classified by TAIR as "pentatricopeptide (PPR) repeat-containing protein". It is located on chr1, region 876259-877548 REVERSE.
Physiological function[]
At1g03510.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g03510.1/Expression
Subcellular localization[]
At1g03510.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g03510.1 has 429 amino-acid residues and a molecular weight Mw = 47.575 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.79, or -0.002 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g03510.1/Knockout
Protein interactions[]
At1g03510.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g03510.1 has 7 recognized domains:
- Domain_0386_0420 PPR domain (PF01535, IPR002885) Description: Pentatricopeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g03510.1
MSSSYASSCT
KLISLTKQLS
SYANQGNHEQ
ALNLFLQMHS
SFALPLDAHV
FSLALKSCAA
AFRPVLGGSV
HAHSVKSNFL
SNPFVGCALL
DMYGKCLSVS
HARKLFDEIP
QRNAVVWNAM
ISHYTHCGKV
KEAVELYEAM
DVMPNESSFN
AIIKGLVGTE
DGSYRAIEFY
RKMIEFRFKP
NLITLLALVS
ACSAIGAFRL
IKEIHSYAFR
NLIEPHPQLK
SGLVEAYGRC
GSIVYVQLVF
DSMEDRDVVA
WSSLISAYAL
HGDAESALKT
FQEMELAKVT
PDDIAFLNVL
KACSHAGLAD
EALVYFKRMQ
GDYGLRASKD
HYSCLVDVLS
RVGRFEEAYK
VIQAMPEKPT
AKTWGALLGA
CRNYGEIELA
EIAARELLMV
EPENPANYVL
LGKIYMSVGR
QEEAERLRLK
MKESGVKVSP
GSSWCLFKD
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.