At1g03687.1

In the plant Arabidopsis thaliana, At1g03687.1 is a gene model for the locus At1g03687.^[1]

This gene model is classified by TAIR as "similar to COG3148: Uncharacterized conserved protein [Yersinia intermedia ATCC 29909] (GB:ZP_00833766.1); similar to Os06g0342600 [Oryza sativa (japonica cultivar-group)] (GB:NP_001057573.1); contains InterPro domain DTW; (InterPro:IPR005636)". It is located on chr1, region 918308-920306 FORWARD.

Physiological function[]

At1g03687.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g03687.1/Expression

Subcellular localization[]

At1g03687.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g03687.1 has 381 amino-acid residues and a molecular weight Mw = 42.922 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.44, or 0.012 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g03687.1/Knockout

Protein interactions[]

At1g03687.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g03687.1 has 1 recognized domains:

Domain_0139_0368 DTW domain (PF03942, IPR005636) Description: DTW_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g03687.1 MRCIAGASVA DFSPSLLFHR RNLVAVGQRL LMTSSILRSH TREFDVCDEG IISVEEWRKW GPVSPFPSAV KQIVDDLKVL ECKLDSPIDF GGNGGKLQGP FGAYEDKKHR ATYEALDDPE KKFRFFSARQ VACRLLGSRG YLCQKCWLAM EDCMCSYVKP CGLWKRIRFW LYMHPRDFLR QNNTGKLLWQ IFGVQSATLC VFGIAEDEEI MWNEFKRAGK SQVRCLYPNH NSEVTFSVKD AFGSSASENH VSSTTDEDKT LHFILLDGTW NNSAAMLKRL KDHAKSVWGD EDLPCISLAT GASAMHKLRP QPSWDRTCTA AAAIGLLSEL SLLPQLSSYE LDKQADAVEE ALVILLDSLT GRRLRMGRSI TRKGRNTISM C ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-