In the plant Arabidopsis thaliana, At1g03750.1 is a gene model for the locus At1g03750.[1]
Also known as SWI2, SNF2, and CHR9, this gene model is classified by TAIR as "CHR9/SNF2/SWI2 (chromatin remodeling 9); helicase". It is located on chr1, region 937919-941067 FORWARD.
Physiological function[]
At1g03750.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g03750.1/Expression
Subcellular localization[]
At1g03750.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g03750.1 has 862 amino-acid residues and a molecular weight Mw = 98.480 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -4.77, or -0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g03750.1/Knockout
Protein interactions[]
At1g03750.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g03750.1 has 3 recognized domains:
- Domain_0142_0480 SNF2_N domain (PF00176, IPR000330) Description: SNF2-related;Molecular Function: DNA binding (GO:0003677), Molecular Function: ATP binding (GO:0005524)
- Domain_0563_0641 Helicase_C domain (PF00271, IPR001650) Description: Helicase, C-terminal;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: helicase activity (GO:0004386), Molecular Function: ATP binding (GO:0005524)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g03750.1
MSLLHTFKET
LKPCGSFPSS
SSLRVSSTQE
LEPSRKPPKS
SLSQQLLRLD
DSYFLPSKHE
SKISKTQVED
FDHNEDDHKR
NIKFDEEEVD
EDDERSIEFG
RPGLSRAEFD
YSGPYEPLML
SSIGEIPIIH
VPASINCRLL
EHQREGVKFM
YNLYKNNHGG
ILGDDMGLGK
TIQTIAFLAA
VYGKDGDAGE
SCLLESDKGP
VLIICPSSII
HNWESEFSRW
ASFFKVSVYH
GSNRDMILEK
LKARGVEVLV
TSFDTFRIQG
PVLSGINWEI
VIADEAHRLK
NEKSKLYEAC
LEIKTKKRIG
LTGTVMQNKI
SELFNLFEWV
APGSLGTREH
FRDFYDEPLK
LGQRATAPER
FVQIADKRKQ
HLGSLLRKYM
LRRTKEETIG
HLMMGKEDNV
VFCQMSQLQR
RVYQRMIQLP
EIQCLVNKDN
PCACGSPLKQ
SECCRRIVPD
GTIWSYLHRD
NHDGCDSCPF
CLVLPCLMKL
QQISNHLELI
KPNPKDEPEK
QKKDAEFVST
VFGTDIDLLG
GISASKSFMD
LSDVKHCGKM
RALEKLMASW
ISKGDKILLF
SYSVRMLDIL
EKFLIRKGYS
FARLDGSTPT
NLRQSLVDDF
NASPSKQVFL
ISTKAGGLGL
NLVSANRVVI
FDPNWNPSHD
LQAQDRSFRY
GQKRHVVVFR
LLSAGSLEEL
VYTRQVYKQQ
LSNIAVAGKM
ETRYFEGVQD
CKEFQGELFG
ISNLFRDLSD
KLFTSDIVEL
HRDSNIDENK
KRSLLETGVS
EDEKEEEVMC
SYKPEMEKPI
LKDLGIVYAH
RNEDIINIGE
TTTSTSQRLN
GDGNSADRKK
KKRKGCSEEE
DMSSSNREQK
REKYKMLAEF
KGMEILEFSR
WVLSASPFDR
EKLLQDFLER
VK
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.