At1g03790.1

In the plant Arabidopsis thaliana, At1g03790.1 is a gene model for the locus At1g03790.^[1]

This gene model is classified by TAIR as "zinc finger (CCCH-type) family protein". It is located on chr1, region 954588-955769 FORWARD.

Physiological function[]

At1g03790.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g03790.1/Expression

Subcellular localization[]

At1g03790.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g03790.1 has 393 amino-acid residues and a molecular weight Mw = 43.617 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.55, or 0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g03790.1/Knockout

Protein interactions[]

At1g03790.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g03790.1 has 2 recognized domains:

Domain_0123_0149 zf-CCCH domain (PF00642, IPR000571) Description: Zinc finger, CCCH-type;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: zinc ion binding (GO:0008270)

Domain_0159_0181 zf-CCCH domain (PF00642, IPR000571) Description: Zinc finger, CCCH-type;Molecular Function: nucleic acid binding (GO:0003676), Molecular Function: zinc ion binding (GO:0008270)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g03790.1 MDVVCTEHQM RKPTVEIPPR KLLLSSKSFP SDSSSPRSPR KHNWNKSNKI TSEHEEDNED NNRENKEYCY DSDSDDPYAS DHFRMFEFKI RRCTRSRSHD WTDCPFAHPG EKARRRDPRR FQYSGEVCPE FRRGGDCSRG DDCEFAHGVF ECWLHPIRYR TEACKDGKHC KRKVCFFAHS PRQLRVLPPE NVSGVSASPS PAAKNPCCLF CSSSPTSTLL GNLSHLSRSP SLSPPMSPAN KAAAFSRLRN RAASAVSAAA AAGSMNYKDV LSELVNSLDS MSLAEALQAS SSSPVTTPVS AAAAAFASSC GLSNQRLHLQ QQQPSSPLQF ALSPSTPSYL TNSPQANFFS DDFTPRRRQM NDFTAMTAVR ENTNIEDGSC GDPDLGWVND LLT ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-