In the plant Arabidopsis thaliana, At1g04140.2 is a gene model for the locus At1g04140.[1]
This gene model is classified by TAIR as "transducin family protein / WD-40 repeat family protein". It is located on chr1, region 1075991-1080320 REVERSE.
Physiological function[]
At1g04140.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g04140.2/Expression
Subcellular localization[]
At1g04140.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g04140.2 has 793 amino-acid residues and a molecular weight Mw = 86.125 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.90, or 0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g04140.2/Knockout
Protein interactions[]
At1g04140.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g04140.2 has 4 recognized domains:
- Domain_0722_0760 WD40 domain (PF00400, IPR001680) Description: WD-40 repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g04140.2
MTESIWLNDQ
SSPRSMIPSA
KPVANVHRQR
CRSVFKLLVQ
REISPNTKFV
PRKRWGESRC
DADSSCGTTS
EPVREQGLNL
ISWVEAESLQ
HLSAKYCPLV
PPPRSTIAAA
FSSDGRTLAS
THGDHTVKII
DCETGKCLKI
LTGHRRTPWV
VRFHPRHSEI
VASGSLDHEV
RLWNAKTGEC
IRTHDFYRPI
ASIAFHAGGE
LLAVASGHKL
HIWHYNKGGD
DSAPAIVLKT
RRSLRAVHFH
PHGVPLLLTA
EVTDIDSSDS
AMTRSTSPGY
LRYPPPAIFF
TNTQSGSRTS
LAAELPLVPL
PYLLLPSYSA
DDPRILYSSG
TTGPRNAQTR
FQSNQSSVEH
GSRTISPSPL
PMATSADLSG
SYHVPDNSAS
NTFATQAGAR
NSTTAVDAMD
VDEAQPVGRN
RVPSQVSSQP
DLLEFGQLQQ
LFHFRDRGSW
ELPFLQGWLM
AQSQAGANSV
ALPTGSSGHV
NSTPYMGSSS
ASHSSTASLE
AGVASLEIPG
GVNLYGVSAR
GDSRDRILQS
RFAGSGLAEG
RSSRNTQHEG
ADAQPVVNRI
PSELASSIAA
AELPCTVKLR
VWSHDIKDPC
SILKSDKCRL
TIHHAVLCSE
MGAHFSPCGR
YLAACVACVI
PHAETDPSLQ
TLVQQDSGLA
TSPTRHPVTA
HQVMYELRVY
SLEKESFGSV
LVSRAIRAAH
CLTSIQFSPT
SEHILLAYGR
RHGSLLKSIV
SDGETTSHFF
TVLEIYRVSD
MELVRVLPSS
EDEVNVACFH
PSPGGGLVYG
TKEGKLRIFR
YNTAAASNLT
APNSSPDENL
AEVELLTRCR
PMR
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.