At1g04620.1

In the plant Arabidopsis thaliana, At1g04620.1 is a gene model for the locus At1g04620.^[1]

This gene model is classified by TAIR as "coenzyme F420 hydrogenase family / dehydrogenase, beta subunit family". It is located on chr1, region 1282868-1286491 REVERSE.

Physiological function[]

At1g04620.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g04620.1/Expression

Subcellular localization[]

At1g04620.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g04620.1 has 462 amino-acid residues and a molecular weight Mw = 51.665 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.09, or 0.009 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g04620.1/Knockout

Protein interactions[]

At1g04620.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g04620.1 has 2 recognized domains:

Domain_0116_0195 FrhB_FdhB_N domain (PF04422, IPR007516) Description: Coenzyme F420 hydrogenase/dehydrogenase beta subunit, N-terminal

Domain_0200_0373 FrhB_FdhB_C domain (PF04432, IPR007525) Description: Coenzyme F420 hydrogenase/dehydrogenase beta subunit, C-terminal_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g04620.1 MITVVTSRLS LLPPVFSVVN SSSSRSKDMN LEPKKKVKLR EDWREKSRPI PPGGTYPAKD HCSQCGLCDT YYIAHVKEAC AFLGDGMSRI ESLEPVVHGR GRKADSLQDT YFGVHQEQLY ARKLKPVEGA QWTGIVTTIA IEMLKSNMVE AVVCVQSDPE DRLSPRPVLA RTPEEVLAAR GVKPTLSPNL NTLELIEASG VKRLLFCGVG CQVQALRSVE QHLNLEKLYV LGTNCVDNGT RDGLDKFLKA ASKEPETVLH YEFMQDYKVQ LKHLDGHIEE VPYFSLPAND LVDVIAPSCY SCFDYTNALA DLVIGYMGVP KYSGLNMTDH PQYITVRNER GKEMLSLVEN LLEITPTISS GDRRPFVTET VKADDAAKFG QGPAQPAPLF VGNIIAFILN LVGPKGLEFA RYSLDYHTIR NYLYVNRKWG KQRANTHMPS YAKKIVEMYN KNGQIDKMLS KK ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-