At1g05140.1

In the plant Arabidopsis thaliana, At1g05140.1 is a gene model for the locus At1g05140.^[1]

This gene model is classified by TAIR as "membrane-associated zinc metalloprotease, putative". It is located on chr1, region 1482680-1484005 FORWARD.

Physiological function[]

At1g05140.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g05140.1/Expression

Subcellular localization[]

At1g05140.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g05140.1 has 441 amino-acid residues and a molecular weight Mw = 47.779 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.78, or 0.011 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g05140.1/Knockout

Protein interactions[]

At1g05140.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g05140.1 has 1 recognized domains:

Domain_0084_0430 Peptidase_M50 domain (PF02163, IPR008915) Description: Peptidase M50;Molecular Function: metalloendopeptidase activity (GO:0004222), Biological Process: proteolysis (GO:0006508)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g05140.1 MLLNISSSPI SHRIPHFLSD FNNPTSNFPP KSKTHLPKSN LSTLSNHSLY GTKNRAFYKN KRNPYNRTQA LGRFDFGSLE SVLEASAVLT AIIVVHETGH FLAASLQGIR VSKFAIGFGP ILAKFNSNNV EYSLRAFPLG GFVGFPDNDP DSDIPVDDRN LLKNRPILDR VIVVSAGIVA NVIFAYAIIF TQVVSVGLPV QESFPGVLVP DVKSFSAASR DGLLPGDVIL AVDGTELSNS GSDSVSKVVD VVKRNPEHNV LLRIERGKES FEIRITPDKS FDGTGKIGVQ LSPNVRFGKV RPKNIPETFS FAGREFFGLS YNVLDSLKQT FLNFSQTASK VAGPVAIIAV GAEVARSNAD GLYQFAALLN LNLAVINLLP LPALDGGTLA LILLEAVRGG RKLPLEVEQG IMSSGIMLVL FLGLFLIVKD TLNLDFIKEM L ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-