At1g05380.1

In the plant Arabidopsis thaliana, At1g05380.1 is a gene model for the locus At1g05380.

This gene model is classified by TAIR as "DNA binding". It is located on chr1, region 1577230-1582189 FORWARD.

Physiological function

At1g05380.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At1g05380.1/Expression

Subcellular localization

At1g05380.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

The gene model At1g05380.1 has 1138 amino-acid residues and a molecular weight Mw = 126.375 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 15.55, or 0.014 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At1g05380.1/Knockout

Protein interactions

At1g05380.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

The gene model At1g05380.1 has 1 recognized domains:

• Domain_0627_0670 PHD domain (PF00628, IPR001965) Description: Zinc finger, PHD-type;Molecular Function: protein binding (GO:0005515), Biological Process: regulation of transcription, DNA-dependent (GO:0006355), Molecular Function: zinc ion binding (GO:0008270)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At1g05380.1
MEGGGGSGER SRIASNTPRS LKEKSDGVGR TVSSTETRQN YQKRSRMVVS DSESSDEFMK PPPRRSGVDR KTLGAKEKFV RKRDRVEHDR NGYVRRNNEA SGSFMKMNKL DIFEFDEYDG FDSANLMRKR FDNGSVGVRG RSSFASRRVD SSVGRSGSGR EGLFDRRRNT FVNGTCSASS QEDSSSESDS DEPMRVQGIN GVLKVKVNNK TNTLAASINP RDAEIYERPP SSRKAQRREN VVVKPPFRKS NNVDNNSESE ESDMSRKSKR KKSEYSKPKK EFNTKSKSTF PELVNPDVRE ERRGRRGGGT DKQRLRERIK GMLTDAGWTI DYKPRRNQSY LDAVYVNPSG TAYWSIIKAY DALLKQLKDE GVDARPRKDT AAVASVSEEI VNKLARKAKK TRSEMTKKWK QNSSGSDSEN KSEGGAYTDT SEERIRSSIK LGGKSTKKGR NGADWDELHK KSKRSLYYNN ARPSCGSDSH YLHGRKTKKI GRCTLLVRSS KDKKNPAING FNPYSGKRTL LSWLIESGVV QLRQKVQYMR RRGAKVMLEG WITREGIHCD CCSKILTVSR FEIHAGSKSC QPFQNIYLES GASLLQCQVR AWNMQKDATN LALHQVDTDG DDPNDDACGI CGDGGDLICC DGCPSTYHQN CLGMQVLPSG DWHCPNCTCK FCDAAVASGG KDGNFISLLS CGMCERRYHQ LCLNDEAHKV QSFGSASSFC GPKCLELFEK LQKYLGVKTE IEGGYSWSLI HRVDTDSDTN SQMSAQRIEN NSKLAVGLAI MDECFLPIVD RRSGVDLIRN VLYNCGSNFN RINYTGFYTA ILERGDEIIS AASLRFHGMQ LAEMPFIGTR HIYRRQGMCR RLFDAIESAM RSLKVEKLVI PAIPDFLHAW TGNFGFTPLD DSVRKEMRSL NTLVFPGIDM LQKPLLHEEN IIAPAAAGDA MISEVETEKK SEFTSSVEIG PYAVEGDEFV ADAANCYKDI LASDEDNILV SVETAMGTIC KPKDELSRHF RGEESGISSS PCQITLKSGT KHVLGHICDD TGSSCEDGLT DVNVEADASL LSQEIQQASA SFKVENNLSL SISGRGSSDL SSISQEVKSE QTSSNLDGVP SCKDYNILVP GAKLDKSKDD AFADGFLL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At1g05380

• PubMed Central references for At1g05380

• Google Scholar references for At1g05380

General database links

• TAIR Locus At1g05380

• TIGR page for At1g05380

• KEGG page for At1g05380

• SWISS-PROT entry for At1g05380

• Structural model of At1g05380

• NCBI structural data for At1g05380

• NCBI Entrez for At1g05380

• Gramene page for At1g05380

• MIPS MAtDB gene page for At1g05380

• MIPS SESAM alignment for At1g05380

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At1g05380

• NCBI protein data for At1g05380

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At1g05380