At1g05610.1

In the plant Arabidopsis thaliana, At1g05610.1 is a gene model for the locus At1g05610.^[1]

Also known as APS2, this gene model is classified by TAIR as "APS2 (ADP-GLUCOSE PYROPHOSHORYLASE SMALL SUBUNIT 2); glucose-1-phosphate adenylyltransferase". It is located on chr1, region 1673894-1675933 REVERSE.

Physiological function[]

At1g05610.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g05610.1/Expression

Subcellular localization[]

At1g05610.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g05610.1 has 476 amino-acid residues and a molecular weight Mw = 53.340 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 5.32, or 0.011 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g05610.1/Knockout

Protein interactions[]

At1g05610.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g05610.1 has 4 recognized domains:

Domain_0056_0324 NTP_transferase domain (PF00483, IPR005835) Description: Nucleotidyl transferase;Biological Process: biosynthesis (GO:0009058), Molecular Function: nucleotidyltransferase activity (GO:0016779)

Domain_0347_0364 Hexapep domain (PF00132, IPR001451) Description: Bacterial transferase hexapeptide repeat

Domain_0369_0386 Hexapep domain (PF00132, IPR001451) Description: Bacterial transferase hexapeptide repeat

Domain_0424_0441 Hexapep domain (PF00132, IPR001451) Description: Bacterial transferase hexapeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g05610.1 MQISSSSFIT KFTNLHMVRS TSDHHQWRHN YNLKQLFIPN LSVSNSQHLP LNQSVAAIVF GGGSDSELYP LTKTRSKGAI PIAANYRLID AVISNCINSG ITKIYAITQF NSTSLNSHLS KAYSGFGLGK DRFVEVIAAY QSLEDQGWFQ GTADAIRRCL WVFEEFPVTE FLVLPGHHLY KMDYKMLIED HRRSRADITI VGLSSVTDHD FGFGFMEVDS TNAVTRFTIK GQQDLISVAN RTATRSDGTS SCSVPSAGIY VIGREQMVKL LRECLIKSKD LASEIIPGAI SEGMKVKAHM FDGYWEDVRS IGAYYRANME SIKSYRFYDR QCPLYTMPRC LPPSSMSVAV ITNSIIGDGC ILDKCVIRGS VVGMRTRIAD EVIVEDSIIV GSDIYEMEED VRRKGKEKKI EIRIGIGEKS RIRRAIVDKN ARIGKNVMII NRDNVEEGNR EAQGYVIREG IIIILRNAVI PNDSIL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-