At1g05690.1

In the plant Arabidopsis thaliana, At1g05690.1 is a gene model for the locus At1g05690.^[1]

Also known as BT3, this gene model is classified by TAIR as "BT3 (BTB and TAZ domain protein 3); protein binding / transcription regulator". It is located on chr1, region 1707808-1709131 FORWARD.

Physiological function[]

At1g05690.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g05690.1/Expression

Subcellular localization[]

At1g05690.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g05690.1 has 364 amino-acid residues and a molecular weight Mw = 41.541 kDa.

The protein is relatively basic; at the physiological pH 7.4, its net charge is 15.99, or 0.044 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g05690.1/Knockout

Protein interactions[]

At1g05690.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g05690.1 has 2 recognized domains:

Domain_0044_0154 BTB domain (PF00651, IPR013069) Description: BTB/POZ

Domain_0229_0321 zf-TAZ domain (PF02135, IPR000197) Description: Zinc finger, TAZ-type;Molecular Function: transcription cofactor activity (GO:0003712), Cellular Component: nucleus (GO:0005634), Biological Process: regulation of transcription, DNA-dependent (GO:0006355), Molecular Function: zinc ion binding (GO:0008270)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g05690.1 MSSSTKNIPK PPPLPCITYQ RFQSSTRKPS SLMRLVPKEA LETWDKLFKE GSGADTYVET DNKSHFPAHS SVLAAASPVI ATLLNQSRDK NGNTYLKIHG VPCEAVYMFI RFLYSSCYEE EEMKKFVLHL LVLSHCYSVP SLKRLCVEIL DQGWINKENV IDVLQLARNC DVTRICFVCL SMVIKDFKSV SSTEGWKVMK RSNPLLEQEL IEAVIESDSR KQERRRKLEE REVYLQLYEA MEALVHICRE GCGTIGPRDK ALKGSHTVCK FPACKGLEGA LRHFLGCKSR ASCSHCKRMW QLLQLHSCIC DDSNSCKVSL CWNFKEKMKK LSKKEQSKWR LLVENIIRAR NSLGPFSSRS SGLI ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-