At1g05805.1

In the plant Arabidopsis thaliana, At1g05805.1 is a gene model for the locus At1g05805.^[1]

This gene model is classified by TAIR as "basic helix-loop-helix (bHLH) family protein". It is located on chr1, region 1744842-1747426 FORWARD.

Physiological function[]

At1g05805.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g05805.1/Expression

Subcellular localization[]

At1g05805.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g05805.1 has 362 amino-acid residues and a molecular weight Mw = 38.951 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.21, or 0.001 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g05805.1/Knockout

Protein interactions[]

At1g05805.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g05805.1 has 1 recognized domains:

Domain_0292_0340 HLH domain (PF00010, IPR001092) Description: Basic helix-loop-helix dimerisation region bHLH;Cellular Component: nucleus (GO:0005634), Molecular Function: transcription regulator activity (GO:0030528), Biological Process: regulation of transcription (GO:0045449)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g05805.1 MYQSSSSTSS SSQRSSLPGG GGLIRYGSAP GSFLNSVVDE VIGGGSSNAR DFTGYQPSSD NFIGNFFTGA ADSSSLRSDS TTCGVNNSSD GQKQLGNNNN NNSNKDIFLD RSYGGFNEIS QQHKSNDIGG GNSSGSYSLA RQRSSPADFF TYLASDKNNF SLNQPTSDYS PQGGSNGGRG HSRLKSQLSF TNHDSLARIN EVNETPVHDG SGHSFSAASF GAATTDSWDD GSGSIGFTVT RPSKRSKDMD SGLFSQYSLP SDTSMNYMDN FMQLPEDSVP CKIRAKRGCA THPRSIAERE RRTRISGKLK KLQDLVPNMD KQTSYSDMLD LAVQHIKGLQ HQLQNLKKDQ ENCTCGCSEK PS ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-