At1g05940.1

In the plant Arabidopsis thaliana, At1g05940.1 is a gene model for the locus At1g05940.^[1]

Also known as CAT9, this gene model is classified by TAIR as "CAT9 (CATIONIC AMINO ACID TRANSPORTER 9); cationic amino acid transporter". It is located on chr1, region 1801364-1803941 REVERSE.

Physiological function[]

At1g05940.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g05940.1/Expression

Subcellular localization[]

At1g05940.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g05940.1 has 569 amino-acid residues and a molecular weight Mw = 60.177 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.86, or 0.002 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g05940.1/Knockout

Protein interactions[]

At1g05940.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g05940.1 has 2 recognized domains:

Domain_0056_0121 AA_permease domain (PF00324, IPR004841) Description: Amino acid permease-associated region;Biological Process: transport (GO:0006810), Cellular Component: membrane (GO:0016020)

Domain_0245_0375 AA_permease domain (PF00324, IPR004841) Description: Amino acid permease-associated region;Biological Process: transport (GO:0006810), Cellular Component: membrane (GO:0016020)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g05940.1 MGGHEGFSNQ RLSSATWFSH FRASALRSKS LPPPSSQTAV RSTSGDSLVR RLGLFDLILL GVGASIGAGV FVVTGTVARD AGPGVTISFL LAGASCVLNA LCYAELSSRF PAVVGGAYMY SYSAFNEITA FLVFVQLMLD YHIGAASISR SLASYAVALL ELFPALKGSI PLWMGSGKEL LGGLLSLNIL APILLALLTL VLCQGVRESS AVNSVMTATK VVIVLVVICA GAFEIDVANW SPFAPNGFKA VLTGATVVFF SYVGFDAVAN SAEESKNPQR DLPIGIMGSL LVCISLYIGV CLVLTGMVPF SLLSEDAPLA EAFSSKGMKF VSILISIGAV AGLTTTLLVG LYVQSRLYLG LGRDGLLPSI FSRIHPTLHT PLHSQIWCGI VAGVLAGIFN VHSLSHILSV GTLTGYSVVA ACVVALRLND KKDRESSNRW TSSWQEGVIC LVIIACSGFG AGVFYRFSAS VIFILLSVGV AVVASAVLHY RQAYALPLGS GFSCPGVPIV PSVCIFFNIF LFAQLHYEAW IRFVVVSVLA TAVYALYGQY HADPSMLDYQ RAPETESDA ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-