At1g06130.1

In the plant Arabidopsis thaliana, At1g06130.1 is a gene model for the locus At1g06130.^[1]

Also known as GLX2-4, this gene model is classified by TAIR as "GLX2-4 (GLYOXALASE 2-4); hydroxyacylglutathione hydrolase". It is located on chr1, region 1858033-1860639 REVERSE.

Physiological function[]

At1g06130.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g06130.1/Expression

Subcellular localization[]

At1g06130.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g06130.1 has 331 amino-acid residues and a molecular weight Mw = 36.628 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.33, or 0.010 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g06130.1/Knockout

Protein interactions[]

At1g06130.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g06130.1 has 1 recognized domains:

Domain_0086_0244 Lactamase_B domain (PF00753, IPR001279) Description: Beta-lactamase-like_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g06130.1 MQAISKVSSA ASFFRCSRKL VSQPCVRPCV RQLHVRKGLV SGVMKLFSSP LRTLRDAGKS VRISRFCSVS NVSSSLQIEL VPCLTDNYAY ILHDEDTGTV GVVDPSEAVP VMDALQKNSR NLTYILNTHH HYDHTGGNLE LKDRYGAKVI GSAADRDRIP GIDVALKDAD KWMFAGHEVH IMETPGHTRG HISFYFPGAR AIFTGDTLFS LSCGKLFEGT PEQMLASLQR IIALPDDTSV YCGHEYTLSN SKFALSIEPT NEVLQSYAAY VAELRDKKLP TIPTTMKMEK ACNPFLRTEN TDIRRALGIP ETADEAEALG IIRRAKDNFK A ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-