In the plant Arabidopsis thaliana, At1g06145.1 is a gene model for the locus At1g06145.[1]
This gene model is classified by TAIR as "binding". It is located on chr1, region 1867128-1868861 REVERSE.
Physiological function[]
At1g06145.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g06145.1/Expression
Subcellular localization[]
At1g06145.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g06145.1 has 577 amino-acid residues and a molecular weight Mw = 65.062 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -5.68, or -0.010 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g06145.1/Knockout
Protein interactions[]
At1g06145.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g06145.1 has 9 recognized domains:
- Domain_0491_0525 PPR domain (PF01535, IPR002885) Description: Pentatricopeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g06145.1
MNAFANVHSL
RVPSHHLRDF
SASLSLAPPN
LKKIIKQCST
PKLLESALAA
MIKTSLNQDC
RLMNQFITAC
TSFKRLDLAV
STMTQMQEPN
VFVYNALFKG
FVTCSHPIRS
LELYVRMLRD
SVSPSSYTYS
SLVKASSFAS
RFGESLQAHI
WKFGFGFHVK
IQTTLIDFYS
ATGRIREARK
VFDEMPERDD
IAWTTMVSAY
RRVLDMDSAN
SLANQMSEKN
EATSNCLING
YMGLGNLEQA
ESLFNQMPVK
DIISWTTMIK
GYSQNKRYRE
AIAVFYKMME
EGIIPDEVTM
STVISACAHL
GVLEIGKEVH
MYTLQNGFVL
DVYIGSALVD
MYSKCGSLER
ALLVFFNLPK
KNLFCWNSII
EGLAAHGFAQ
EALKMFAKME
MESVKPNAVT
FVSVFTACTH
AGLVDEGRRI
YRSMIDDYSI
VSNVEHYGGM
VHLFSKAGLI
YEALELIGNM
EFEPNAVIWG
ALLDGCRIHK
NLVIAEIAFN
KLMVLEPMNS
GYYFLLVSMY
AEQNRWRDVA
EIRGRMRELG
IEKICPGTSS
IRIDKRDHLF
AAADKSHSAS
DEVCLLLDEI
YDQMGLAGYV
QETENVY
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.