At1g06240.1

In the plant Arabidopsis thaliana, At1g06240.1 is a gene model for the locus At1g06240.^[1]

This gene model is classified by TAIR as "oxidoreductase/ transition metal ion binding". It is located on chr1, region 1911165-1912874 FORWARD.

Physiological function[]

At1g06240.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g06240.1/Expression

Subcellular localization[]

At1g06240.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g06240.1 has 383 amino-acid residues and a molecular weight Mw = 42.265 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -2.88, or -0.008 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g06240.1/Knockout

Protein interactions[]

At1g06240.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g06240.1 has 1 recognized domains:

Domain_0090_0345 DUF455 domain (PF04305, IPR007402) Description: Protein of unknown function DUF455_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g06240.1 MLIHHLRPFS TASLSPRSLT ISTALSSSST KQQHKLWSGL ENWRKSPVND LRLWGPTGPL LPSSSDSISA DFYGLVSAAS SLADLGALVL STSDPLSKSH ISHLAFSRWR RENLPVGSIS HLPSSPARPP KPLLVATNQV PNPKDSNLPL NAHMLHNLAH VELNAIDLAW DTVARFSPFF DLLGHNFFDD FAHVADDESR HFLWCSQRLA ELGFKYGDIP ANNLLMRECE KTSNNVAARL ACIPLVQEAR GLDAGPRLVK RLTGFGDNRT SKIVAKIAEE EVAHVAVGVD WFLSVCQKMN RAPSPTFKDL IKEYGVELRG PFNHSAREVA GIPRDWYDPS CGTEVDKGDN EQGDKEQLSA VYDRLTHIIS MESENSSLEK PAK ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-