At1g06730.1

In the plant Arabidopsis thaliana, At1g06730.1 is a gene model for the locus At1g06730.^[1]

This gene model is classified by TAIR as "pfkB-type carbohydrate kinase family protein". It is located on chr1, region 2067632-2070048 FORWARD.

Physiological function[]

At1g06730.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g06730.1/Expression

Subcellular localization[]

At1g06730.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g06730.1 has 488 amino-acid residues and a molecular weight Mw = 52.754 kDa.

The protein is relatively acidic; at the physiological pH 7.4, its net charge is -8.22, or -0.017 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g06730.1/Knockout

Protein interactions[]

At1g06730.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g06730.1 has 2 recognized domains:

Domain_0131_0174 PfkB domain (PF00294, IPR011611) Description: PfkB

Domain_0224_0411 PfkB domain (PF00294, IPR011611) Description: PfkB_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g06730.1 MYHHAFSLKF HSFSPHYALS LSNFSTQSPN PLLPPFARVP HVTSSVCLRC RSSAADVSPV IYADGSSSIC SFGETGDVAV VEKPIDVSTL GNLCVDIVLS VHELPPPSRG ERKALMDELS MSPPDKKYWE AGGNCNMAIA AARLGLHCVA IGHVGDEIYG EFLLDVLHEE GIGTVALNGG TNEKDTSSFC ETLICWVLVD PLQRHGFCSR ADFKEEPAFS WITDLSDEVK MAIRQSKVLF CNGYDFDDFS PSFIMSTIDY AAKVGTAIFF DPGPRGKSLS KGTPDERRAL AHFLRMSDVL LLTSEEVEAL TGIRNPVKAG QEILRNGKGT KWVIVKMGPK GSILVTKSSV SVAPAFKVEV VDTVGCGDSF VAAIALGYIR NMPLVNTLTI ANAVGAATAM GCGAGRNVAK RHQVVDLMKA SKLNDEEKFF EQLLAENSES SRINLLSKGM IKDGRSNKQL IETISMEKVV SELLAELELG RCCVKASS ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-