At1g07705.2

In the plant Arabidopsis thaliana, At1g07705.2 is a gene model for the locus At1g07705.^[1]

This gene model is classified by TAIR as "similar to VIP2 (VIRE2 INTERACTING PROTEIN2), transcription regulator [Arabidopsis thaliana] (TAIR:AT5G59710.1); similar to NOT2/NOT3/NOT5 [Medicago truncatula] (GB:ABE93791.1); similar to NOT2/NOT3/NOT5 family protein, expressed [Oryza sativa (japonica cultivar-group)] (GB:ABF98742.1); similar to VIP2 [Nicotiana benthamiana] (GB:AAY15746.1); contains InterPro domain NOT2/NOT3/NOT5; (InterPro:IPR007282)". It is located on chr1, region 2382087-2385853 FORWARD.

Physiological function[]

At1g07705.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g07705.2/Expression

Subcellular localization[]

At1g07705.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g07705.2 has 614 amino-acid residues and a molecular weight Mw = 65.815 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 1.33, or 0.002 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g07705.2/Knockout

Protein interactions[]

At1g07705.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g07705.2 has 1 recognized domains:

Domain_0466_0603 NOT2_3_5 domain (PF04153, IPR007282) Description: NOT2/NOT3/NOT5;Cellular Component: nucleus (GO:0005634), Molecular Function: transcription regulator activity (GO:0030528), Biological Process: regulation of transcription (GO:0045449)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g07705.2 MSSLLNSSIN GSTSNLSDGS GRTFTSSFSG QSGAASPVFH HAGSIQGLHN IHGNFNVPNL AGSLGSRNSS LNGVPSAGVQ QQNGSISNGR FASSNIPVAL SQMSHGSSHG HSGLTNRGGL GVSPILGNVG SRMTSSMGNM VGGGTMGRTL SSGGGLSIPS LGSRLNLAVN SGSGNIGQNR MMGGVLPQGS PQVLSMLGNS YPSAGGLSQN HVQAMNSLSS MGLLNDMNSN DTSPFDINND FPQLTSRPSS AGSQGQLGSR LKQGLGISPI VQQNQEFSIQ NEDFPALPGY KGSSADYPMD LHHKEQLHEN SVLMMQSQQL SMGRSGGFNL GGAYTSHRPQ QQQQHAQAVS SSGVSLHGSD IFSSSHPPYH SQTGGAPGIG LRSMNSANSI TGMGYDQQLI QQYQHQQNSA QYRLQQMSAA SQPFRDVGLK SMQSTQSNPD RFGLLGLLSV IKMSDPDLTS LALGIDLTTL GLNLNSTENL HKTFGSPWSN EPSKVDPEFS VPQCYYAKNP PPLHQGLFAK LLVETLFYVF YSMPKDEAQL YAANELYNRG WFYHKEHRLW FIRIGEPLVK TNAYERGSYH CFDPNSFEIV QKENFVLYYE MLEKRPSISQ DSQH ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-