At1g07910.1

In the plant Arabidopsis thaliana, At1g07910.1 is a gene model for the locus At1g07910.^[1]

Also known as ATRNL, and RNL, this gene model is classified by TAIR as "ATRNL/RNL (ARABIDOPSIS THALIANA RNA LIGASE); 2',3'-cyclic-nucleotide 3'-phosphodiesterase/ RNA ligase (ATP)/ polynucleotide kinase". It is located on chr1, region 2446709-2454383 FORWARD.

Physiological function[]

At1g07910.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g07910.1/Expression

Subcellular localization[]

At1g07910.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g07910.1 has 1104 amino-acid residues and a molecular weight Mw = 123.208 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 6.00, or 0.005 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g07910.1/Knockout

Protein interactions[]

At1g07910.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g07910.1 has no known domains._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g07910.1 MDAPFESGDS SATVVAEAVN NQFGGLSLKE SNTNAPVLPS QTTSNHRVQN LVWKPKSYGT VSGSSSATEV GKTSAVSQIG SSGDTKVGLN LSKIFGGNLL EKFSVDKSTY CHAQIRATFY PKFENEKTDQ EIRTRMIEMV SKGLATLEVS LKHSGSLFMY AGHKGGAYAK NSFGNIYTAV GVFVLSRMFR EAWGTKAPKK EAEFNDFLEK NRMCISMELV TAVLGDHGQR PLDDYVVVTA VTELGNGKPQ FYSTSEIISF CRKWRLPTNH VWLFSTRKSV TSFFAAFDAL CEEGIATSVC RALDEVADIS VPASKDHVKV QGEILEGLVA RIVSSQSSRD MENVLRDHPP PPCDGANLDL GLSLREICAA HRSNEKQQMR ALLRSVGPSF CPSDVEWFGD ESHPKSADKS VITKFLQSQP ADYSTSKLQE MVRLMKEKRL PAAFKCYHNF HRAEDISPDN LFYKLVVHVH SDSGFRRYHK EMRHMPSLWP LYRGFFVDIN LFKSNKGRDL MALKSIDNAS ENDGRGEKDG LADDDANLMI KMKFLTYKLR TFLIRNGLSI LFKDGAAAYK TYYLRQMKIW GTSDGKQKEL CKMLDEWAAY IRRKCGNDQL SSSTYLSEAE PFLEQYAKRS PKNHILIGSA GNLVRTEDFL AIVDGDLDEE GDLVKKQGVT PATPEPAVKE AVQKDEGLIV FFPGIPGSAK SALCKELLNA PGGFGDDRPV HTLMGDLVKG KYWPKVADER RKKPQSIMLA DKNAPNEDVW RQIEDMCRRT RASAVPIVAD SEGTDTNPYS LDALAVFMFR VLQRVNHPGK LDKESSNAGY VLLMFYHLYE GKNRNEFESE LIERFGSLIK MPLLKSDRTP LPDPVKSVLE EGIDLFNLHS RRHGRLESTK GTYAAEWTKW EKQLRDTLVA NSEYLSSIQV PFESMVHQVR EELKTIAKGD YKPPSSEKRK HGSIVFAAIN LPATQVHSLL EKLAAANPTM RSFLEGKKKS IQEKLERSHV TLAHKRSHGV ATVASYSQHL NREVPVELTE LIYNDKMAAL TAHVGSVDGE TVVSKNEWPH VTLWTAEGVT AKEANTLPQL YLEGKASRLV IDPPVSISGP LEFF ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-