In the plant Arabidopsis thaliana, At1g08070.1 is a gene model for the locus At1g08070.[1]
This gene model is classified by TAIR as "pentatricopeptide (PPR) repeat-containing protein". It is located on chr1, region 2514371-2516596 REVERSE.
Physiological function[]
At1g08070.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g08070.1/Expression
Subcellular localization[]
At1g08070.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g08070.1 has 741 amino-acid residues and a molecular weight Mw = 83.505 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -4.68, or -0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g08070.1/Knockout
Protein interactions[]
At1g08070.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g08070.1 has 10 recognized domains:
- Domain_0507_0541 PPR domain (PF01535, IPR002885) Description: Pentatricopeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g08070.1
MMLSCSPLTV
PSSSYPFHFL
PSSSDPPYDS
IRNHPSLSLL
HNCKTLQSLR
IIHAQMIKIG
LHNTNYALSK
LIEFCILSPH
FEGLPYAISV
FKTIQEPNLL
IWNTMFRGHA
LSSDPVSALK
LYVCMISLGL
LPNSYTFPFV
LKSCAKSKAF
KEGQQIHGHV
LKLGCDLDLY
VHTSLISMYV
QNGRLEDAHK
VFDKSPHRDV
VSYTALIKGY
ASRGYIENAQ
KLFDEIPVKD
VVSWNAMISG
YAETGNYKEA
LELFKDMMKT
NVRPDESTMV
TVVSACAQSG
SIELGRQVHL
WIDDHGFGSN
LKIVNALIDL
YSKCGELETA
CGLFERLPYK
DVISWNTLIG
GYTHMNLYKE
ALLLFQEMLR
SGETPNDVTM
LSILPACAHL
GAIDIGRWIH
VYIDKRLKGV
TNASSLRTSL
IDMYAKCGDI
EAAHQVFNSI
LHKSLSSWNA
MIFGFAMHGR
ADASFDLFSR
MRKIGIQPDD
ITFVGLLSAC
SHSGMLDLGR
HIFRTMTQDY
KMTPKLEHYG
CMIDLLGHSG
LFKEAEEMIN
MMEMEPDGVI
WCSLLKACKM
HGNVELGESF
AENLIKIEPE
NPGSYVLLSN
IYASAGRWNE
VAKTRALLND
KGMKKVPGCS
SIEIDSVVHE
FIIGDKFHPR
NREIYGMLEE
MEVLLEKAGF
VPDTSEVLQE
MEEEWKEGAL
RHHSEKLAIA
FGLISTKPGT
KLTIVKNLRV
CRNCHEATKL
ISKIYKREII
ARDRTRFHHF
RDGVCSCNDY
W
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.