In the plant Arabidopsis thaliana, At1g08130.1 is a gene model for the locus At1g08130.[1]
Also known as ATLIG1, this gene model is classified by TAIR as "ATLIG1 (ARABIDOPSIS THALIANA DNA LIGASE 1); ATP binding / DNA ligase (ATP)". It is located on chr1, region 2542910-2547812 REVERSE.
Physiological function[]
At1g08130.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g08130.1/Expression
Subcellular localization[]
At1g08130.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g08130.1 has 790 amino-acid residues and a molecular weight Mw = 87.743 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.66, or 0.005 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g08130.1/Knockout
Protein interactions[]
At1g08130.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g08130.1 has 3 recognized domains:
- Domain_0175_0353 DNA_ligase_A_N domain (PF04675, IPR012308) Description: DNA ligase, N-terminal;Molecular Function: DNA binding (GO:0003677), Molecular Function: DNA ligase (ATP) activity (GO:0003910), Biological Process: DNA replication (GO:0006260), Biological Process: DNA repair (GO:0006281), Biological Process: DNA recombination (GO:0006310)
- Domain_0418_0622 DNA_ligase_A_M domain (PF01068, IPR012310) Description: ATP dependent DNA ligase, central;Molecular Function: DNA ligase (ATP) activity (GO:0003910), Molecular Function: ATP binding (GO:0005524), Biological Process: DNA replication (GO:0006260), Biological Process: DNA repair (GO:0006281), Biological Process: DNA recombination (GO:0006310)
- Domain_0647_0712 DNA_ligase_A_C domain (PF04679, IPR012309) Description: ATP dependent DNA ligase, C-terminal;Molecular Function: DNA ligase (ATP) activity (GO:0003910), Biological Process: DNA replication (GO:0006260), Biological Process: DNA repair (GO:0006281), Biological Process: DNA recombination (GO:0006310)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g08130.1
MLAIRSSNYL
RCIPSLCTKT
QISQFSSVLI
SFSRQISHLR
LSSCHRAMSS
SRPSAFDALM
SNARAAAKKK
TPQTTNLSRS
PNKRKIGETQ
DANLGKTIVS
EGTLPKTEDL
LEPVSDSANP
RSDTSSIAED
SKTGAKKAKT
LSKTDEMKSK
IGLLKKKPND
FDPEKMSCWE
KGERVPFLFV
ALAFDLISNE
SGRIVITDIL
CNMLRTVIAT
TPEDLVATVY
LSANEIAPAH
EGVELGIGES
TIIKAISEAF
GRTEDHVKKQ
NTELGDLGLV
AKGSRSTQTM
MFKPEPLTVV
KVFDTFRQIA
KESGKDSNEK
KKNRMKALLV
ATTDCEPLYL
TRLLQAKLRL
GFSGQTVLAA
LGQAAVYNEE
HSKPPPNTKS
PLEEAAKIVK
QVFTVLPVYD
IIVPALLSGG
VWNLPKTCNF
TLGVPIGPML
AKPTKGVAEI
LNKFQDIVFT
CEYKYDGERA
QIHFMEDGTF
EIYSRNAERN
TGKYPDVALA
LSRLKKPSVK
SFILDCEVVA
FDREKKKILP
FQILSTRARK
NVNVNDIKVG
VCIFAFDMLY
LNGQQLIQEN
LKIRREKLYE
SFEEDPGYFQ
FATAVTSNDI
DEIQKFLDAS
VDVGCEGLII
KTLDSDATYE
PAKRSNNWLK
LKKDYMDSIG
DSVDLVPIAA
FHGRGKRTGV
YGAFLLACYD
VDKEEFQSIC
KIGTGFSDAM
LDERSSSLRS
QVIATPKQYY
RVGDSLNPDV
WFEPTEVWEV
KAADLTISPV
HRAATGIVDP
DKGISLRFPR
LLRVREDKKP
EEATSSEQIA
DLYQAQKHNH
PSNEVKGDDD
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.