At1g08840.1

In the plant Arabidopsis thaliana, At1g08840.1 is a gene model for the locus At1g08840.

Also known as EMB2411, this gene model is classified by TAIR as "EMB2411 (EMBRYO DEFECTIVE 2411); ATP-dependent DNA helicase". It is located on chr1, region 2829582-2838372 REVERSE.

Physiological function

At1g08840.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At1g08840.1/Expression

Subcellular localization

At1g08840.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

The gene model At1g08840.1 has 1296 amino-acid residues and a molecular weight Mw = 143.241 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -5.54, or -0.004 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At1g08840.1/Knockout

Protein interactions

At1g08840.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

The gene model At1g08840.1 has 1 recognized domains:

• Domain_0888_0945 UvrD-helicase domain (PF00580, IPR000212) Description: UvrD/REP helicase;Molecular Function: DNA binding (GO:0003677), Molecular Function: ATP-dependent DNA helicase activity (GO:0004003), Molecular Function: ATP binding (GO:0005524), Biological Process: DNA repair (GO:0006281)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At1g08840.1
MPPRKKPKSS ALKSNKQSSA NHSSQPSTFG IQQLFLRHIQ NSQSTSNSHT STADPVDQQN VNGLASDTAV LTPQNPLGTS NEKPDESKDM DQQLTEASPK ISKNLKRFSP GMLIKQSQDD CGGEITWKIS PVNERLRAAA KNIPKMMDLT ENSLGKQCPT SGITSKVEQW LSSPSKKASK RPAFATNRVM ERVNPSPDAE FEIVNTSSSG NSPFQTPPSL SCPHNKLPCT VTCSGACGSM GAGQHKKALL ELLDQVEDVI AVDDKTTDDV GIVMPQARVK DDIISSVVDC AVDEGPVSLP KMQNSINPDS YFLVLEVSEK RGSGSSSKGQ CPYKVLRLLD EHTGVECALY LWDEWFYSTV SPGDSINVIG EFDGDGKCDV DRQNNFLIVH PDTLVAGTRV AASFGCPRRT VLDERLRSNE HATVALLGTL QHQVFQAGLS QESPSVDGLQ EYASTVIEKS IESLYACGVH EGDVRSTLFK AIPKMLNWIE HFRYSKDSEV SKVDFGSTIG KKAVKVSEVI DIEEMSWAPK YGLKGMIDAS VRVIVESDMN TVNEKIMPLE FKSGKAPSGQ SSIEHSAQVI LYTLLMSERY LKHIDNGLLY YLQSDQTQGI SVQRSDLVGL IIRRNELAND ILVASTTQQL PPMLRADGGN TESSGLGDVF DTHVSHLSTL HFNFLRHWDR LIDLEGREMQ LLRKDIAHPH GSKGSHSASY LSSMVLDVTN GFQHHNSHKE TRFIYRFVRQ KSSESRERVT SEDMIRTGNL ATDDLDCKLR TGDRVILRTE VSHLTVANGI IADISRTHIS VSLSKRLRLP WSEPSSEVSN LSHELWRIYK DEFMTSFSVM RFNLMQLFVQ NGHNIRKMIV DLEPPRFDNG SILSQDPAIS YIWSEKSLNN DQRQAILKIL TAKDYALILG MPGTGKTSTM VHAVKALLIR GSSILLASYT NSAVDNLLIK LKAQGIEFLR IGRDEAVHEE VRESCFSAMN MCSVEDIKKK LDQVKVVAST CLGINSPLLV NRRFDVCIID EAGQIALPVS IGPLLFASTF VLVGDHYQLP PLVQSTEARE NGMGISLFRR LSEAHPQAIS VLQNQYRMCR GIMELSNALI YGDRLCCGSA EVADATLVLS TSSSTSPWLK KVLEPTRTVV FVNTDMLRAF EARDQNAINN PVEASIIAEI VEELVNNGVD SKDIGIITPY NSQASLIQHA IPTTPVEIHT IDKYQGRDKD CILVSFVRSR EKPRSSASSL LGDWHRINVA LTRAKKKLIM VGSQRTLSRV PLLMLLLNKV KEQSGILNLL PGDLKP ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At1g08840

• PubMed Central references for At1g08840

• Google Scholar references for At1g08840

General database links

• TAIR Locus At1g08840

• TIGR page for At1g08840

• KEGG page for At1g08840

• SWISS-PROT entry for At1g08840

• Structural model of At1g08840

• NCBI structural data for At1g08840

• NCBI Entrez for At1g08840

• Gramene page for At1g08840

• MIPS MAtDB gene page for At1g08840

• MIPS SESAM alignment for At1g08840

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At1g08840

• NCBI protein data for At1g08840

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At1g08840