At1g09130.2

In the plant Arabidopsis thaliana, At1g09130.2 is a gene model for the locus At1g09130.^[1]

This gene model is classified by TAIR as "endopeptidase Clp". It is located on chr1, region 2940065-2942219 REVERSE.

Physiological function[]

At1g09130.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g09130.2/Expression

Subcellular localization[]

At1g09130.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g09130.2 has 330 amino-acid residues and a molecular weight Mw = 36.310 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.21, or 0.010 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g09130.2/Knockout

Protein interactions[]

At1g09130.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g09130.2 has 1 recognized domains:

Domain_0124_0306 CLP_protease domain (PF00574, IPR001907) Description: Peptidase S14, ClpP;Biological Process: proteolysis (GO:0006508), Molecular Function: endopeptidase Clp activity (GO:0008462)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g09130.2 MASCLQASMN SLLPRSSSFS PHPPLSSNSS GRRNLKTFRY AFRAKASAKI PMPPINPKDP FLSTLASIAA NSPEKLLNRP VNADVPPYLD IFDSPQLMSS PAQVERSVAY NEHRPRTPPP DLPSMLLDGR IVYIGMPLVP AVTELVVAEL MYLQWLDPKE PIYIYINSTG TTRDDGETVG MESEGFAIYD SLMQLKNEVH TVCVGAAIGQ ACLLLSAGTK GKRFMMPHAK AMIQQPRVPS SGLMPASDVL IRAKEVITNR DILVELLSKH TGNSVETVAN VMRRPYYMDA PKAKEFGVID RILWRGQEKI IADVVPSEEF DKNAGIKSVV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-