At1g10390.2

In the plant Arabidopsis thaliana, At1g10390.2 is a gene model for the locus At1g10390.

This gene model is classified by TAIR as "transporter". It is located on chr1, region 3407266-3412046 REVERSE.

Physiological function

At1g10390.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression

At1g10390.2/Expression

Subcellular localization

At1g10390.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties

The gene model At1g10390.2 has 1041 amino-acid residues and a molecular weight Mw = 106.312 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 5.78, or 0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype

At1g10390.2/Knockout

Protein interactions

At1g10390.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure

The gene model At1g10390.2 has 1 recognized domains:

• Domain_0869_1041 Nucleoporin2 domain (PF04096, IPR007230) Description: Peptidase S59, nucleoporin;Cellular Component: nuclear pore (GO:0005643), Biological Process: transport (GO:0006810)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence

>At1g10390.2
MFGSSNPFGQ SSGTSPFGSQ SLFGQTSNTS SNNPFAPATP FGTSAPFAAQ SGSSIFGSTS TGVFGAPQTS SPFASTPTFG ASSSPAFGNS TPAFGASPAS SPFGGSSGFG QKPLGFSTPQ SNPFGNSTQQ SQPAFGNTSF GSSTPFGATN TPAFGAPSTP SFGATSTPSF GASSTPAFGA TNTPAFGASN SPSFGATNTP AFGASPTPAF GSTGTTFGNT GFGSGGAFGA SNTPAFGASG TPAFGASGTP AFGASSTPAF GASSTPAFGA SSTPAFGGSS TPSFGASNTS SFSFGSSPAF GQSTSAFGSS AFGSTPSPFG GAQASTPTFG GSGFGQSTFG GQQGGSRAVP YAPTVEADTG TGTQPAGKLE SISAMPAYKE KNYEELRWED YQRGDKGGPL PAGQSPGNAG FGISPSQPNP FSPSPAFGQT SANPTNPFSS STSTNPFAPQ TPTIASSSFG TATSNFGSSP FGVTSSSNLF GSGSSTTTSV FGSSSAFGTT TPSPLFGSSS TPGFGSSSSI FGSAPGQGAT PAFGNSQPST LFNSTPSTGQ TGSAFGQTGS AFGQFGQSSA PAFGQNSIFN KPSTGFGNMF SSSSTLTTSS SSPFGQTMPA GVTPFQSAQP GQASNGFGFN NFGQNQAANT TGNAGGLGIF GQGNFGQSPA PLNSVVLQPV AVTNPFGTLP AMPQISINQG GNSPSIQYGI SSMPVVDKPA PVRISSLLTS RHLLHRRVRL PARKYRPGEN GPKVPFFTDD EESSSTPKAD ALFIPRENPR ALVIRPVQQW SSRDKSILPK EQRPTAPLHD NGKSPDMATD AANHDRNGNG ELGATGERIH TSVNANQKPN GTTRSDQASE KERPYKTLSG HRAGEAAIVY EHGADIEALM PKLRQSDYFT EPRIQELAAK ERADPGYCRR VRDFVVGRHG YGSIKFMGET DVRRLDLESL VQFNTREVIV YMDESKKPAV GQGLNKPAEV TLLNIKCIDK KTGKQFTEGE RVEKYKMMLK KKAEAQGAEF VSFDPVKGEW KFRVEHFSSY KLGDEDEEDG V ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-

Multiple sequence alignment

A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.

Protein structure

A predicted structure for this protein is available here.

Further reading

• PubMed references for At1g10390

• PubMed Central references for At1g10390

• Google Scholar references for At1g10390

General database links

• TAIR Locus At1g10390

• TIGR page for At1g10390

• KEGG page for At1g10390

• SWISS-PROT entry for At1g10390

• Structural model of At1g10390

• NCBI structural data for At1g10390

• NCBI Entrez for At1g10390

• Gramene page for At1g10390

• MIPS MAtDB gene page for At1g10390

• MIPS SESAM alignment for At1g10390

• MIPS PENDANT general properties

• MIPS PENDANT general sequence alignment

• NCBI gene data for At1g10390

• NCBI protein data for At1g10390

• NCBI location on chromosome

• SUBA subcellular localization

• Arabidopsis MPSS Plus on At1g10390