At1g10410.1

In the plant Arabidopsis thaliana, At1g10410.1 is a gene model for the locus At1g10410.^[1]

This gene model is classified by TAIR as "similar to CW14 [Arabidopsis thaliana] (TAIR:AT1G59650.1); similar to expressed protein [Oryza sativa (japonica cultivar-group)] (GB:ABF98316.1); contains InterPro domain Protein of unknown function DUF1336; (InterPro:IPR009769)". It is located on chr1, region 3416843-3419265 REVERSE.

Physiological function[]

At1g10410.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g10410.1/Expression

Subcellular localization[]

At1g10410.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g10410.1 has 485 amino-acid residues and a molecular weight Mw = 54.876 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.44, or 0.009 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g10410.1/Knockout

Protein interactions[]

At1g10410.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g10410.1 has 1 recognized domains:

Domain_0232_0473 DUF1336 domain (PF07059, IPR009769) Description: Protein of unknown function DUF1336_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g10410.1 MGGCVSTPKS CVGAKLRSSK RRKSRRRRKI QRKRAAVSSR LSDGSFDNLD HHHRNFSNPS SRATGEDAWF ESNVAFETDC DDDFHSVHED ALSLNGSERV SLSSTTTTSS TRDTDSNEVM SQSKSDGDLN DTNQPDLIDS SADEGLLENC RILPSNCLPC LNTTTVPSID KRRSLSSSPP SSRKKSSLRL SYKWREGHAS GALFLSKMQL KRPIAGSQVP FCPIDKKMLD CWSTIDPNSF RVRGKTYLRE KKKEFAPSHA AYNPFGVDVF LSEHKIHHVA QYVKLPVTTT STKLPSILVV NVQIPLYPTA IFQGESDGEG MNIVLYFKLS DNYSKELPLH FQESIRRLID DEVEKVKGFP LDTTAPFRER LKILGRVANV DDLHLSGPEK KLMQAYNEKP VLSRPQHEFY LGDNYFEIDI DMHRFGYISR KGFETFIDRL KICVLDVGLT IQGNKPEELP EQILCCVRLN GIDFMNYHQL TQELL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-