At1g10510.1

In the plant Arabidopsis thaliana, At1g10510.1 is a gene model for the locus At1g10510.^[1]

Also known as EMB2004, this gene model is classified by TAIR as "EMB2004 (EMBRYO DEFECTIVE 2004); protein binding". It is located on chr1, region 3461772-3465591 FORWARD.

Physiological function[]

At1g10510.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g10510.1/Expression

Subcellular localization[]

At1g10510.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g10510.1 has 605 amino-acid residues and a molecular weight Mw = 64.722 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.78, or 0.001 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g10510.1/Knockout

Protein interactions[]

At1g10510.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g10510.1 has 1 recognized domains:

Domain_0339_0365 LRR_1 domain (PF00560, IPR001611) Description: Leucine-rich repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g10510.1 MASSSTSSLN LHSLPKASSG LGQWKSGFRY ELLGSSVSRN RLFVSPVVIH HRSPRLPAIK AAYNSDGGSK RSRVYKESQA ASGFPNAKVQ QIASSVLPLG SFAVVTFVLW KVVGKFMSPK SPKTSAGENN SSTQGVKWSI GAGTNLLQGF AAKVDREAKQ RLNEFAKELR SFRSVDMSGC NFGDEGLFFL AESLGYNQTV EEVSFSANGI TAAGVKAFDG VLQSNIMLKI LNLSGNPIGD EGAKTLCATL MENSSIEILQ LNSTDIGDEG AKEIAELLKR NSTLRIIELN NNMIDYSGFT SLAGALLENN TIRNLHLNGN YGGALGANAL AKGLEGNKSL RELHLHGNSI GDEGTRALMA GLSSHKGKVA LLDLGNNSIS AKGAFYVAEY IKRSKSLVWL NLYMNDIGDE GAEKIADSLK QNRSIATIDL GGNNIHAEGV NAIAQALKDN AIITTLEVGY NPIGPDGAKA LSEILKFHGN VKTLKLGWCQ IAAKGAEHVA DMLRYNNTIS VLDLRANGLR DEGASCLARS LKVVNEALTS VDLGFNEIRD DGAFAIAQAL KANEDVTVTS INLGNNFITK FGQSALTDAR DHVLEMTEKE VEIFF ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-