In the plant Arabidopsis thaliana, At1g11290.1 is a gene model for the locus At1g11290.[1]
This gene model is classified by TAIR as "pentatricopeptide (PPR) repeat-containing protein". It is located on chr1, region 3791454-3793883 REVERSE.
Physiological function[]
At1g11290.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g11290.1/Expression
Subcellular localization[]
At1g11290.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g11290.1 has 809 amino-acid residues and a molecular weight Mw = 90.870 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 4.65, or 0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g11290.1/Knockout
Protein interactions[]
At1g11290.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g11290.1 has 12 recognized domains:
- Domain_0576_0609 PPR domain (PF01535, IPR002885) Description: Pentatricopeptide repeat_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g11290.1
MSSQLVQFST
VPQIPNPPSR
HRHFLSERNY
IPANVYEHPA
ALLLERCSSL
KELRQILPLV
FKNGLYQEHF
FQTKLVSLFC
RYGSVDEAAR
VFEPIDSKLN
VLYHTMLKGF
AKVSDLDKAL
QFFVRMRYDD
VEPVVYNFTY
LLKVCGDEAE
LRVGKEIHGL
LVKSGFSLDL
FAMTGLENMY
AKCRQVNEAR
KVFDRMPERD
LVSWNTIVAG
YSQNGMARMA
LEMVKSMCEE
NLKPSFITIV
SVLPAVSALR
LISVGKEIHG
YAMRSGFDSL
VNISTALVDM
YAKCGSLETA
RQLFDGMLER
NVVSWNSMID
AYVQNENPKE
AMLIFQKMLD
EGVKPTDVSV
MGALHACADL
GDLERGRFIH
KLSVELGLDR
NVSVVNSLIS
MYCKCKEVDT
AASMFGKLQS
RTLVSWNAMI
LGFAQNGRPI
DALNYFSQMR
SRTVKPDTFT
YVSVITAIAE
LSITHHAKWI
HGVVMRSCLD
KNVFVTTALV
DMYAKCGAIM
IARLIFDMMS
ERHVTTWNAM
IDGYGTHGFG
KAALELFEEM
QKGTIKPNGV
TFLSVISACS
HSGLVEAGLK
CFYMMKENYS
IELSMDHYGA
MVDLLGRAGR
LNEAWDFIMQ
MPVKPAVNVY
GAMLGACQIH
KNVNFAEKAA
ERLFELNPDD
GGYHVLLANI
YRAASMWEKV
GQVRVSMLRQ
GLRKTPGCSM
VEIKNEVHSF
FSGSTAHPDS
KKIYAFLEKL
ICHIKEAGYV
PDTNLVLGVE
NDVKEQLLST
HSEKLAISFG
LLNTTAGTTI
HVRKNLRVCA
DCHNATKYIS
LVTGREIVVR
DMQRFHHFKN
GACSCGDYW
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.