At1g12350.1

In the plant Arabidopsis thaliana, At1g12350.1 is a gene model for the locus At1g12350.^[1]

Also known as ATCOAB, this gene model is classified by TAIR as "ATCOAB (4-PHOSPHO-PANTO-THENOYLCYSTEINE SYNTHETASE); catalytic/ phosphopantothenate--cysteine ligase". It is located on chr1, region 4198868-4200631 FORWARD.

Physiological function[]

At1g12350.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g12350.1/Expression

Subcellular localization[]

At1g12350.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g12350.1 has 317 amino-acid residues and a molecular weight Mw = 35.369 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 0.65, or 0.002 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g12350.1/Knockout

Protein interactions[]

At1g12350.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g12350.1 has 1 recognized domains:

Domain_0043_0289 DFP domain (PF04127, IPR007085) Description: DNA/pantothenate metabolism flavoprotein, C-terminal_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g12350.1 MSSISGLVED EISSFFESSP PLKNKEEIVA NLNQFIELNS SCQGGMRRIV CVTSGGTTVP LEQRCVRYID NFSSGNRGAA STENFVKAGY AVIFLYRRGT CQPYCRYLPD DPFLECFEFP DAKNIQVHGS HSGAVKMAVM DQQAAVAEGR LLKLPFSTIY EYLQMLRLIA TILKDVGPCS MFYLAAAVSD FYVPWLSMTE HKIESGSGPL DIRLAQVPKM LSILRSNWAP KAFCISFKLE TDSKILLEKA TKALQKYKVH AVVANELLTR KEEVVVVSSS GNVVVRRDSN KPESIVEDNL IRLLVDRHST YIKESLT ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-