In the plant Arabidopsis thaliana, At1g12430.1 is a gene model for the locus At1g12430.[1]
Also known as PAK, this gene model is classified by TAIR as "PAK (PHOSPHATIDIC ACID KINASE); microtubule motor". It is located on chr1, region 4234120-4238550 REVERSE.
Physiological function[]
At1g12430.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g12430.1/Expression
Subcellular localization[]
At1g12430.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g12430.1 has 919 amino-acid residues and a molecular weight Mw = 100.878 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is -6.74, or -0.007 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g12430.1/Knockout
Protein interactions[]
At1g12430.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g12430.1 has 4 recognized domains:
- Domain_0076_0413 Kinesin domain (PF00225, IPR001752) Description: Kinesin, motor region;Molecular Function: microtubule motor activity (GO:0003777), Molecular Function: ATP binding (GO:0005524), Cellular Component: microtubule associated complex (GO:0005875), Biological Process: microtubule-based movement (GO:0007018)
- Domain_0774_0814 Arm domain (PF00514, IPR000225) Description: Armadillo_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g12430.1
MSTTSGTGGV
SYRNGTQRSS
LRTQSSASTS
SGGQKASVKS
KSVLRKSSPA
ALGGGSSKSG
GGGDAGVPGR
VRVAVRLRPR
NGEELIADAD
FADCVELQPE
LKRLKLRKNN
WDTDTFEFDE
VLTEYASQKR
VYEVVAKPVV
EGVLDGYNGT
IMAYGQTGTG
KTYTLGQLGE
EDVADRGIMV
RAMEDILAEV
SLETDSISVS
YLQLYMETVQ
DLLDPSNDNI
AIVEDPKNGD
VSLPGATLVE
IRDQQSFLEL
LQLGEAHRFA
ANTKLNTESS
RSHAILMVNV
RRSMKTRDGL
SSESNGNSHM
TKSLKPPVVR
KGKLVVVDLA
GSERINKSGS
EGHTLEEAKS
INLSLSALGK
CINALAENSS
HVPFRDSKLT
RLLRDSFGGT
ARTSLVITIG
PSPRHRGETT
STIMFGQRAM
KVENMVKIKE
EFDYKSLSRR
LEVQLDNLIE
ENERQQKAFV
DEIERITVEA
HNQISEAEKR
YANALEDEKL
RYQNDYMESI
KKLEENWSKN
QKKLAAERLA
LGEKNGLDIT
SNGNRSIAPA
LEEVSELKKL
LQKEAQSKMA
AEEEVNRLKH
QLNEFKKVEA
SGNSEIMRLH
KMLENETQQK
EKLEGEIATL
HSQLLQLSLT
ADETRRNLEQ
HGSEKTSGAR
DSLMSQLRLP
QIQDPGNAEK
PPVARLFEQV
GLQKILSLLE
AEDADVRIHA
VKVVANLAAE
EANQQQIVEA
GGLTSLLMLL
KNTEDETIHR
VAAGAIANLA
MNETNQELIM
DQGGIGLLSS
TAANAEDPQT
LRMVAGAIAN
LCGNDKLQTK
LRSEGGIAAL
LGMVRCGHPD
VLAQVARGIA
NFAKCESRAS
TQGTKRGKSL
LIEDGALSWI
VQNAKTETAA
IRRHIELALC
HLAQHEGNAK
EMVKEGAMWE
LVRISRDCSR
EDIRSLAHRT
LTSSPTFLTE
LRRLRVDIR
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.