At1g12520.1

In the plant Arabidopsis thaliana, At1g12520.1 is a gene model for the locus At1g12520.^[1]

Also known as ATCCS, and CCS1, this gene model is classified by TAIR as "CCS1 (copper chaperone for superoxide dismutase 1); superoxide dismutase copper chaperone". It is located on chr1, region 4267275-4268898 REVERSE.

Physiological function[]

At1g12520.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g12520.1/Expression

Subcellular localization[]

At1g12520.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g12520.1 has 320 amino-acid residues and a molecular weight Mw = 33.848 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -3.34, or -0.010 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g12520.1/Knockout

Protein interactions[]

At1g12520.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g12520.1 has 2 recognized domains:

Domain_0090_0150 HMA domain (PF00403, IPR006121) Description: Heavy metal transport/detoxification protein;Biological Process: metal ion transport (GO:0030001), Molecular Function: metal ion binding (GO:0046872)

Domain_0161_0290 Sod_Cu domain (PF00080, IPR001424) Description: Superoxide dismutase, copper/zinc binding;Molecular Function: copper, zinc superoxide dismutase activity (GO:0004785), Biological Process: superoxide metabolism (GO:0006801), Molecular Function: metal ion binding (GO:0046872)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g12520.1 MASILRSVAT TSAVVAAASA IPIAIAFSSS SSSSSTNPKS QSLNFSFLSR SSPRLLGLSR SFVSSPMATA LTSDRNLHQE DRAMPQLLTE FMVDMTCEGC VNAVKNKLET IEGIEKVEVD LSNQVVRILG SSPVKAMTQA LEQTGRKARL IGQGVPQDFL VSAAVAEFKG PDIFGVVRFA QVSMELARIE ANFTGLSPGT HSWCINEYGD LTNGAASTGS LYNPFQDQTG TEPLGDLGTL EADKNGEAFY SGKKEKLKVA DLIGRAVVVY KTDDNKSGPG LTAAVIARSA GVGENYKKLC SCDGTVIWEA TNSDFVASKV ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-