In the plant Arabidopsis thaliana, At1g12860.1 is a gene model for the locus At1g12860.[1]
This gene model is classified by TAIR as "basic helix-loop-helix (bHLH) family protein / F-box family protein". It is located on chr1, region 4382221-4386390 FORWARD.
Physiological function[]
At1g12860.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.
Tissue expression[]
At1g12860.1/Expression
Subcellular localization[]
At1g12860.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Biophysical properties[]
The gene model At1g12860.1 has 828 amino-acid residues and a molecular weight Mw = 93.381 kDa.
The protein is relatively neutral; at the physiological pH 7.4, its net charge is 7.00, or 0.008 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.
Knockout phenotype[]
At1g12860.1/Knockout
Protein interactions[]
At1g12860.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.
Domain structure[]
The gene model At1g12860.1 has 2 recognized domains:
- Domain_0644_0691 HLH domain (PF00010, IPR001092) Description: Basic helix-loop-helix dimerisation region bHLH;Cellular Component: nucleus (GO:0005634), Molecular Function: transcription regulator activity (GO:0030528), Biological Process: regulation of transcription (GO:0045449)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.
Amino-acid sequence[]
>At1g12860.1
MESREDSFIS
KEKKSTMKKE
KQAIASQRNR
RRVIKNRGNG
KRLIASLSQR
KRRRIPRGRG
NEKAVFAPSS
LPNDVVEEIF
LRLPVKAIIQ
LKSLSKQWRS
TIESRSFEER
HLKIVERSRV
DFPQVMVMSE
EYSLKGSKGN
QPRPDTDIGF
STICLESASI
LSSTLITFPQ
GFQHRIYASE
SCDGLFCIHS
LKTQAIYVVN
PATRWFRQLP
PARFQILMQK
LYPTQDTWID
IKPVVCYTAF
VKANDYKLVW
LYNSDASNPN
LGVTKCEVFD
FRANAWRYLT
CTPSYRIFPD
QVPAATNGSI
YWFTEPYNGE
IKVVALDIHT
ETFRVLPKIN
PAIASSDPDH
IDMCTLDNGL
CMSKRESDTL
VQEIWRLKSS
EDSWEKFDMN
SDGVWLDGSG
ESPEVNNGEA
ASWVRNPDED
WFNNPPPPQH
TNQNDFRFNG
GFPLNPSENL
LLLLQQSIDS
SSSSSPLLHP
FTLDAASQQQ
QQQQQQQEQS
FLATKACIVS
LLNVPTINNN
TFDDFGFDSG
FLGQQFHGNH
QSPNSMNFTG
LNHSVPDFLP
APENSSGSCG
LSPLFSNRAK
VLKPLQVMAS
SGSQPTLFQK
RAAMRQSSSS
KMCNSESSSE
MRKSSYEREI
DDTSTGIIDI
SGLNYESDDH
NTNNNKGKKK
GMPAKNLMAE
RRRRKKLNDR
LYMLRSVVPK
ISKMDRASIL
GDAIDYLKEL
LQRINDLHTE
LESTPPSSSS
LHPLTPTPQT
LSYRVKEELC
PSSSLPSPKG
QQPRVEVRLR
EGKAVNIHMF
CGRRPGLLLS
TMRALDNLGL
DVQQAVISCF
NGFALDVFRA
EQCQEDHDVL
PEQIKAVLLD
TAGYAGLV
________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________
MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE
WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ
FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP
CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF
MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP
VLNVFLSHGISQIRSRY-
Multiple sequence alignment[]
A full-length multiple-sequence alignment (MSA) is available here. This MSA may be visualized by submitting it to a server such as ESPript.
Protein structure[]
A predicted structure for this protein is available here.
References[]
- ↑ This is a test reference.