At1g12900.1

In the plant Arabidopsis thaliana, At1g12900.1 is a gene model for the locus At1g12900.^[1]

Also known as GAPA-2, this gene model is classified by TAIR as "GAPA-2; glyceraldehyde-3-phosphate dehydrogenase". It is located on chr1, region 4392632-4394281 REVERSE.

Physiological function[]

At1g12900.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g12900.1/Expression

Subcellular localization[]

At1g12900.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g12900.1 has 399 amino-acid residues and a molecular weight Mw = 42.848 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.10, or 0.005 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g12900.1/Knockout

Protein interactions[]

At1g12900.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g12900.1 has 2 recognized domains:

Domain_0065_0216 Gp_dh_N domain (PF00044, IPR000173) Description: Glyceraldehyde 3-phosphate dehydrogenase;Molecular Function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity (GO:0004365), Biological Process: glycolysis (GO:0006096), Molecular Function: NAD binding (GO:0051287)

Domain_0221_0377 Gp_dh_C domain (PF02800, IPR000173) Description: Glyceraldehyde 3-phosphate dehydrogenase;Molecular Function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity (GO:0004365), Biological Process: glycolysis (GO:0006096), Molecular Function: NAD binding (GO:0051287)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g12900.1 MASATFSVAK PSLQGFSEFS GLRNSSALPF AKRSSSDEFV SFVSFQTSAM RSNGGYRKGV TEAKIKVAIN GFGRIGRNFL RCWHGRKDSP LDVVVINDTG GVKQASHLLK YDSTLGIFDA DVKPSGDSAL SVDGKIIKIV SDRNPSNLPW GELGIDLVIE GTGVFVDRDG AGKHLQAGAK KVLITAPGKG DIPTYVVGVN AELYSHEDTI ISNASCTTNC LAPFVKVLDQ KFGIIKGTMT TTHSYTGDQR LLDASHRDLR RARAAALNIV PTSTGAAKAV ALVLPNLKGK LNGIALRVPT PNVSVVDLVV QVSKKTFAEE VNAAFRDAAE KELKGILDVC DEPLVSVDFR CSDVSSTIDS SLTMVMGDDM VKVIAWYDNE WGYSQRVVDL ADIVANNWK ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-