At1g13270.1

In the plant Arabidopsis thaliana, At1g13270.1 is a gene model for the locus At1g13270.^[1]

Also known as MAP1B, and MAP1C, this gene model is classified by TAIR as "MAP1C (METHIONINE AMINOPEPTIDASE 1B); metalloexopeptidase". It is located on chr1, region 4544996-4547152 FORWARD.

Physiological function[]

At1g13270.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g13270.1/Expression

Subcellular localization[]

At1g13270.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g13270.1 has 369 amino-acid residues and a molecular weight Mw = 40.424 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -0.01, or -0.000 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g13270.1/Knockout

Protein interactions[]

At1g13270.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g13270.1 has 1 recognized domains:

Domain_0133_0368 Peptidase_M24 domain (PF00557, IPR000994) Description: Peptidase M24;Biological Process: proteolysis (GO:0006508), Molecular Function: metalloexopeptidase activity (GO:0008235)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g13270.1 MASSVFLSSF SSSSSLQLCS SFHGEYLAPS RCFLGAPVTS SSLSLSGKKN SYSPRQFHVS AKKVSGLEEA IRIRKMRELE TKSKVRRNPP LRRGRVSPRL LVPDHIPRPP YVESGVLPDI SSEFQIPGPE GIAKMRAACE LAARVLNYAG TLVKPSVTTN EIDKAVHDMI IEAGAYPSPL GYGGFPKSVC TSVNECMCHG IPDSRQLQSG DIINIDVTVY LDGYHGDTSR TFFCGEVDEG FKRLVKVTEE CLERGIAVCK DGASFKKIGK RISEHAEKFG YNVVERFVGH GVGPVFHSEP LIYHYRNDEP GLMVEGQTFT IEPILTIGTT ECVTWPDNWT TLTADGGVAA QFEHTILITR TGSEILTKC ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-