At1g13350.1

In the plant Arabidopsis thaliana, At1g13350.1 is a gene model for the locus At1g13350.^[1]

This gene model is classified by TAIR as "protein kinase family protein". It is located on chr1, region 4572499-4576544 REVERSE.

Physiological function[]

At1g13350.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g13350.1/Expression

Subcellular localization[]

At1g13350.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g13350.1 has 761 amino-acid residues and a molecular weight Mw = 87.173 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is -2.64, or -0.003 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g13350.1/Knockout

Protein interactions[]

At1g13350.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g13350.1 has 1 recognized domains:

Domain_0442_0758 Pkinase domain (PF00069, IPR000719) Description: Protein kinase;Molecular Function: protein kinase activity (GO:0004672), Molecular Function: ATP binding (GO:0005524), Biological Process: protein amino acid phosphorylation (GO:0006468)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g13350.1 MVSDKHVESN HRKHRRSFSP SDEVFKSPKR HKSRHHHRRH GHRHHRDEEV QYNDDENVNG GDLDMEEGEI LGKEGIGETL KKKLESVDEF GDIKSGQFRE NNLGRNQRRE RECEKRKEIE PDRERRKERG SVDRDSRGDR EKDYLRDRDN DRGRSRDKAR YSSRERGREN ERERRSEKDR DKGREFQSDR EKHKSLDDGY GEVRHKHSGH SRHDAEDDLE LRSPTSVNGH DPNSGDVKET RGNVERTRID NDDKGDVVVW EVEQEDEELN LIEESRRRTQ AIMEKYKKKL EQQNGFSSHD LELANIPKQS STVADVLGSG TLGPVTSAVN QAKAGLDIDA VDGEVAKLSS AVGESPAQLV ISDSDRTLAS TGLGEGSPKD KISDDMFTDD IFGESPADSQ KMGYLRGKGN GIPIVRSGLD DNWDDAEGYY SYQLGELLDD RYEIMATHGK GVFSTVVRAK DTKAELGEPE EVAIKIIRNN ETMHKAGQTE IQILKKLAGS DPENKRHCVR FLSTFKYRNH LCLVFESLHL NLREIVKKYG RNIGIQLSGV RVYATQLFIS LKHLKNCGVL HCDIKPDNML VNEGRNTLKL CDFGSAMFAG TNEVTPYLVS RFYRAPEIIL GLPYDHPLDI WSVGCCLYEL FSGKIMFPGS TNNEMLRLHM ELKGAFPKKM LRKGAFIDQH FDKDLCFYAT EEDSVTRKTT KRMMVNIKPK EFGSVIKQRY KDEDSKLLVH FRDLLDRIFI LDPQKRITVS QALAHPFITG K ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-