At1g13460.1

In the plant Arabidopsis thaliana, At1g13460.1 is a gene model for the locus At1g13460.^[1]

This gene model is classified by TAIR as "serine/threonine protein phosphatase 2A (PP2A) regulatory subunit B', putative". It is located on chr1, region 4616501-4618177 FORWARD.

Physiological function[]

At1g13460.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g13460.1/Expression

Subcellular localization[]

At1g13460.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g13460.1 has 492 amino-acid residues and a molecular weight Mw = 56.514 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 5.45, or 0.011 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g13460.1/Knockout

Protein interactions[]

At1g13460.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g13460.1 has 1 recognized domains:

Domain_0072_0485 B56 domain (PF01603, IPR002554) Description: Protein phosphatase 2A, regulatory B subunit, B56;Cellular Component: protein phosphatase type 2A complex (GO:0000159), Biological Process: signal transduction (GO:0007165), Molecular Function: protein phosphatase type 2A regulator activity (GO:0008601)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g13460.1 MWKQILSKLP KKSSSKNHSS SSSSTSKSSD NGASKSGNSQ TQNAPPVKPS ADSGFKEGNL KGNGNGFTPY EALPGFKDVP NAEKQNLFVR KLSLCCVVFD FSDPTKNVKE KDIKRQTLLE LVDYVASPNG KFSETVIQEV VRMVSVNIFR TLNPQPRENK VIDALDLEEE EPSMDPTWPH LQLVYEILLR LIASPETDTK LAKKYIDQSF VSRLLDLFDS EDPRERDCLK TVLHRIYGKF MVHRPFIRKS INNIFYRFVF ETEKHNGIAE FLEILGSIIN GFALPLKDEH KVFLVRALVP LHKPKSLQMY HQQLSYCITQ FVEKDCKLAD TVIRGLLKSW PVTNSSKEVM FLNELEEVLE ATQPPEFQRC MVPLFRQVAR CLNSLHFQVA ERALFLWNND HIENLIMQNR KVILPIIFPA LERNTQKHWN QAVHSLTLNV QKIFNDIDAE LFKDCLAKFR EDESKEAEIG AKREATWKRL EEIGNQKQKS SL ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-