At1g13960.2

In the plant Arabidopsis thaliana, At1g13960.2 is a gene model for the locus At1g13960.^[1]

Also known as WRKY4, this gene model is classified by TAIR as "WRKY4 (WRKY DNA-binding protein 4); transcription factor". It is located on chr1, region 4776619-4779040 FORWARD.

Physiological function[]

At1g13960.2/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g13960.2/Expression

Subcellular localization[]

At1g13960.2/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g13960.2 has 487 amino-acid residues and a molecular weight Mw = 53.052 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 2.66, or 0.005 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g13960.2/Knockout

Protein interactions[]

At1g13960.2/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g13960.2 has 2 recognized domains:

Domain_0201_0259 WRKY domain (PF03106, IPR003657) Description: DNA-binding WRKY;Molecular Function: transcription factor activity (GO:0003700), Cellular Component: nucleus (GO:0005634), Molecular Function: sequence-specific DNA binding (GO:0043565), Biological Process: regulation of transcription (GO:0045449)

Domain_0381_0440 WRKY domain (PF03106, IPR003657) Description: DNA-binding WRKY;Molecular Function: transcription factor activity (GO:0003700), Cellular Component: nucleus (GO:0005634), Molecular Function: sequence-specific DNA binding (GO:0043565), Biological Process: regulation of transcription (GO:0045449)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g13960.2 MSEKEEAPST SKSTGAPSRP TLSLPPRPFS EMFFNGGVGF SPGPMTLVSN MFPDSDEFRS FSQLLAGAMS SPATAAAAAA AATASDYQRL GEGTNSSSGD VDPRFKQNRP TAVLDLICNI VQGSYGMTHQ QALAQVTAQA VQANANMQPQ TEYPPPSQVQ SFSSGQAQIP TSAPLPAQRE TSDVTIIEHR SQQPLNVDKP ADDGYNWRKY GQKQVKGSEF PRSYYKCTNP GCPVKKKVER SLDGQVTEII YKGQHNHEPP QNTKRGNKDN TANINGSSIN NNRGSSELGA SQFQTNSSNK TKREQHEAVS QATTTEHLSE ASDGEEVGNG ETDVREKDEN EPDPKRRSTE VRISEPAPAA SHRTVTEPRI IVQTTSEVDL LDDGYRWRKY GQKVVKGNPY PRSYYKCTTP GCGVRKHVER AATDPKAVVT TYEGKHNHDL PAAKSSSHAA AAAQLRPDNR PGGLANLNQQ QQQQPVARLR LKEEQTT ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-