At1g13970.1

In the plant Arabidopsis thaliana, At1g13970.1 is a gene model for the locus At1g13970.^[1]

This gene model is classified by TAIR as "similar to unknown protein [Arabidopsis thaliana] (TAIR:AT3G29180.1); similar to unknown protein [Oryza sativa (japonica cultivar-group)] (GB:AAO38509.1); similar to Os12g0577600 [Oryza sativa (japonica cultivar-group)] (GB:NP_001067115.1); similar to expressed protein [Oryza sativa (japonica cultivar-group)] (GB:ABF98316.1); contains InterPro domain Protein of unknown function DUF1336; (InterPro:IPR009769)". It is located on chr1, region 4779739-4781879 REVERSE.

Physiological function[]

At1g13970.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g13970.1/Expression

Subcellular localization[]

At1g13970.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g13970.1 has 504 amino-acid residues and a molecular weight Mw = 57.598 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 5.21, or 0.010 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g13970.1/Knockout

Protein interactions[]

At1g13970.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g13970.1 has 1 recognized domains:

Domain_0245_0489 DUF1336 domain (PF07059, IPR009769) Description: Protein of unknown function DUF1336_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g13970.1 MGGCVSSQRK LSNKLQQKKQ KRGRSGKCRS KISASMPDVP MKRMSNASVR DFAVSEYVHL DFDNCAAKMM CKRAEMSNAN FHLTQLQWNC SQIDGNRISH EEAWYDSFSY IDSDSDDGSN SSVFEDANAS AMGQVIQYEE FYESYLKIDG NKGETYSSKN EVSIKRNQVA DESHHETFKT TTCEDHQDHR KKSSKVVMVS VRRTSIDSKS TPSEFSSAEK LLYRPKAGSM IQRSLGEKMT SQGSWSEVSP SSFKLRGLNF FRDKQKCPAP NCSPYIPIGV DLFACPKKIN HIAQHIELPN LKPASSQVCD IPNLLIVNIQ LPMYPTSMFG DYDGEGLSLV LYFKRNENYH KEISSHFKET IKRFMEDEME KVKGFTREST VPFRERLKIM AGLVNPEDFQ LSSTERKLIT AYNDRPVLSR PQHDFFQGPN YFEIDLDIHR FSYISRKGLE SFRDRIKNGI LDLGLTIQAQ SPEELPEQVL CCVRLNKIDF VNHGQIPTLL TNKQ ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-