At1g14230.1

In the plant Arabidopsis thaliana, At1g14230.1 is a gene model for the locus At1g14230.^[1]

This gene model is classified by TAIR as "nucleoside phosphatase family protein / GDA1/CD39 family protein". It is located on chr1, region 4861492-4863986 FORWARD.

Physiological function[]

At1g14230.1/Function_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ ______________ ________________ (At1g78590) NADK3 (At1g78590) is a NAD(H) kinase with a strong preference for NADH over NAD+. NAD(H) kinases phosphorylate NAD(H) with ATP (other phosphoryl donors are not used preferentially) to form NADP(H). Phosphorylation of NAD(H) is the sole known means of generating NADP(H) de novo.

Tissue expression[]

At1g14230.1/Expression

Subcellular localization[]

At1g14230.1/Localization_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Biophysical properties[]

The gene model At1g14230.1 has 503 amino-acid residues and a molecular weight Mw = 55.200 kDa.

The protein is relatively neutral; at the physiological pH 7.4, its net charge is 3.10, or 0.006 per residue._____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ NADK3-GFP has been shown by in vivo transient expression to be localized to the cytosol, confirmed by immunoblotting.

Knockout phenotype[]

At1g14230.1/Knockout

Protein interactions[]

At1g14230.1/Interactions_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ This protein does not interact with calmodulin in the presence or absence of calcium or EGTA. NADK3 has been reported to be phosphorylated in vitro by AtMAPK4. NADK3 forms a homodimer.

Domain structure[]

The gene model At1g14230.1 has 1 recognized domains:

Domain_0072_0494 GDA1_CD39 domain (PF01150, IPR000407) Description: Nucleoside phosphatase GDA1/CD39;Molecular Function: hydrolase activity (GO:0016787)_____________The entry below does not belong to this gene_________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________

NADK3 contains the conserved NAD(H) kinase domain, GSTAAMQSAGG for binding NAD and the upstream GGDGT ATP-binding domain.

Amino-acid sequence[]

>At1g14230.1 MQRSNARSRS NINSDMVDPP EVQTSPGNHR SSPSTAAKPK SKRTKSIIFV IVACVTIALG LLFIGYSILR SGRNRRVSLH YSVIIDGGSS GTRVHVFGYR IESGKPVFDF GEENYASLKL SPGLSAYADN PEGVSESVTE LVEFAKKRVH KGKLKKSDIR LMATAGMRLL ELPVQEQILD VTRRVLRSSG FDFRDEWASV ISGSDEGVYA WVVANHALGS LGGEPLKTTG IVELGGASAQ VTFVSTELVP SEFSRTLAYG NVSYNLYSHS FLDFGQDAAQ EKLSESLYNS AANSTGEGIV PDPCIPKGYI LETNLQKDLP GFLADKGKFT ATLQAAGNFS ECRSAAFAML QEEKGKCTYK RCSIGSIFTP NLQGSFLATE NFFHTSKFFG LGEKEWLSEM ILAGKRFCGE EWSKLKVKYP TFKDENLLRY CFSSAYIISM LHDSLGVALD DERIKYASKA GEEDIPLDWA LGAFILNTAT ATFDYSGKSR KILDLSNVAK YKI ________________Given the above incorrect annotations, this may, or may not, be the correct sequence for At1g57680.1 _________________ ___________________________ ____________________ ___________ __________ __________________ __________________ _________________ _________________ MAIRKLLLLLKPIDPYPFLQTEGASLIKNPQVLQYLESRCKVHKNAIKFCQEILSKKPVE WKPISRNDLSHPIRDVDMVITVGGDGTLLHASHFIDDSVPVLGVNSDPTQAHEVEELSDQ FDASRSTGHLCAATVENFEQVLDDILFGRVVPAKVSRISLKLNSETLLSHALNDILIAQP CPAAVSRFSFKIKNKDGASSPKTVNCRSSGLRICTAAGSTAAMQSAGGFVMPMLSRDLQF MVREPISPGSTASLMHSTFKPDQFMDVNWYSDHGTIYIDGCQVQHSVQLGDTIEISSDAP VLNVFLSHGISQIRSRY-